Laboratory Animal and Comparative Medicine ›› 2003, Vol. 23 ›› Issue (2): 67-69.

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Isolation, Culture and Identification of Porcine Bone Marrow Mesenchymal Stem Cells and Transformation to Myogenic Cells in vitro

  

  1. 1.Department of Cardio-T horacic Surgery, Renji Hospital，Shanghai Second Medical University， Shanghai 200001，China；2.Department of Clinical Laboratory, Collage of Technique, Jiangsu University，Zhenjiang 212001，China；3.Department of Cardio-Thoracic Surgery，Xinhua Hospital,shanghai Second Medical University，Shanghai 200082，China；4.Department of Pathology，Shanghai Second Medical University，Shanghai 200025，China
  • Received:2002-07-15 Online:2003-01-25 Published:2013-03-19

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Abstract: To establish a method of isolating and obtaining porcine bone marrow mesenchymal stem cells （MSCs）in vitro，and to assess the possibility of MSCs as a new ccll source for tissue engineering. Three ml bone marrow was extracted from the posterior superior iliac spine of the mini-pig and culturd by 1×106/ml cells. Some cells were attached to the culture dish after the second day.The adherent cells approached confluence 10 days after plating and then every passage was cultured for 5～7 days. The primary culture and 10th passaged adherent cells were induced to differentiate into osteoblast and myogenic cells. The primary culture and the passaged cells were spindle-shaped in appearance.They both might be induced to differentiate into bone marrow mesenchymal cells and osteoblast and myogenic cells under special environment. The method for isolating and culturing porcine MSCs was feasible.

Key words: Bone marrow mesenchymal stem cells, Cell culture, Ostoblast, Myogenic cells