Abstract: Objective To investigate the effect of bone marrow mesencymals stem cells transplantation on the deep partial thickness derma burn injury in hamsters and explore the possible mechanism. Methods Fourty hamsters were used to create deep partial thickness derma burn injury model and randomly divided into two groups: transplantation group (group A) and control group (group B). The wound of animals in group A were treated with local transplantation of the fifth generation cells of MSCs cultured in vitro with concentration of 1×10⁷ /ml plus surface daub of supernatant. The group B were treated with the same amount of saline. The wound healing effect was observed by HE staining, chymase mRNA level was analyzed on day 1, 3, 7, 14 after transplantation. Results The HE staining results showed the quality of healing was better in transplantation group than in control group. PCR results showed that chymase gene expression was found in both groups with the highest expression on day 3.Conclusion BMMSCs transplantations can promote wound healing, and dermal mastocyte chymotrysin would be involved in wound healing process.

Key words: Bone marrow mesenchymal stem cells, Burn, Hamster, Chymase