Laboratory Animal and Comparative Medicine ›› 2024, Vol. 44 ›› Issue (2): 227-233.DOI: 10.12300/j.issn.1674-5817.2023.101
• Facilities and Management for Laboratory Animals • Previous Articles
Lina PENG()(
), Man ZHANG, Min AI, Wangjie XU(
)(
)
Received:
2023-07-12
Revised:
2023-10-18
Online:
2024-04-25
Published:
2024-05-09
Contact:
Wangjie XU
CLC Number:
Lina PENG, Man ZHANG, Min AI, Wangjie XU. Discussion on Management Experience of Public Cell Culture Platform in Laboratory Animal Center of Shanghai Jiao Tong University[J]. Laboratory Animal and Comparative Medicine, 2024, 44(2): 227-233.
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URL: https://www.slarc.org.cn/dwyx/EN/10.12300/j.issn.1674-5817.2023.101
设备维护项目 | 每次 | 每天 | 每月 | 每季度 | 每半年 | 每年 |
---|---|---|---|---|---|---|
Maintenance projects | Each time | Daily | Monthly | Per quarter | Semiannually | Per annum |
初效过滤器Primary efficiency filter | ② | ③ | ||||
中效过滤器Medium efficiency filter | ③ | |||||
高效过滤器High efficiency filter | ③ | |||||
传递窗中紫外灯Ultraviolet lamp in delivery window | ① | ③ | ||||
生物安全柜中紫外灯Ultraviolet lamp in biosafety cabinet | ① | ③* | ||||
细胞培养室中紫外灯Ultraviolet lamp in cell culture room | ① | ③ | ||||
落下菌检测Airborne bacteria detection | ④ | |||||
尘埃粒子检测Dust particle detection | ④ |
Table 1 Maintenance frequency of hardware facilities in cell culture laboratories of Laboratory Animal Center of Shanghai Jiao Tong University
设备维护项目 | 每次 | 每天 | 每月 | 每季度 | 每半年 | 每年 |
---|---|---|---|---|---|---|
Maintenance projects | Each time | Daily | Monthly | Per quarter | Semiannually | Per annum |
初效过滤器Primary efficiency filter | ② | ③ | ||||
中效过滤器Medium efficiency filter | ③ | |||||
高效过滤器High efficiency filter | ③ | |||||
传递窗中紫外灯Ultraviolet lamp in delivery window | ① | ③ | ||||
生物安全柜中紫外灯Ultraviolet lamp in biosafety cabinet | ① | ③* | ||||
细胞培养室中紫外灯Ultraviolet lamp in cell culture room | ① | ③ | ||||
落下菌检测Airborne bacteria detection | ④ | |||||
尘埃粒子检测Dust particle detection | ④ |
消毒物品或位置 | 每次 | 每天 | 每周 | 每月 |
---|---|---|---|---|
Sterilized items or locations | Each time | Daily | Weekly | Monthly |
实验室台面Laboratory countertop | ②③ | ①④ | ||
生物安全柜台面Biosecurity countertop | ①②③ | |||
仪器操作台面Instrument operating table | ② | ①④ | ||
细胞培养室Cell culture room | ①④ | |||
传递窗Delivery window | ①③ | ② | ||
进出口缓冲间Inlet and outlet buffer | ①③④ | |||
冰箱Icebox | ② | |||
二氧化碳培养箱内部及水盘Carbon dioxide incubator inside and water tray | ② | |||
置物架Storage shelf | ①④ | ② | ||
储物柜Locker | ①④ | ② | ||
门 Door | ①④ | ② | ||
墙壁 Wall | ①④ | ② | ||
天花板 Ceiling | ①④ | ② |
Table 2 Frequency of cleaning and disinfection in cell culture laboratories of Laboratory Animal Center of Shanghai Jiao Tong University
消毒物品或位置 | 每次 | 每天 | 每周 | 每月 |
---|---|---|---|---|
Sterilized items or locations | Each time | Daily | Weekly | Monthly |
实验室台面Laboratory countertop | ②③ | ①④ | ||
生物安全柜台面Biosecurity countertop | ①②③ | |||
仪器操作台面Instrument operating table | ② | ①④ | ||
细胞培养室Cell culture room | ①④ | |||
传递窗Delivery window | ①③ | ② | ||
进出口缓冲间Inlet and outlet buffer | ①③④ | |||
冰箱Icebox | ② | |||
二氧化碳培养箱内部及水盘Carbon dioxide incubator inside and water tray | ② | |||
置物架Storage shelf | ①④ | ② | ||
储物柜Locker | ①④ | ② | ||
门 Door | ①④ | ② | ||
墙壁 Wall | ①④ | ② | ||
天花板 Ceiling | ①④ | ② |
实验物品 Experimental item | 高压灭菌 Autoclave | 化学消毒 | 物理消毒 | |
---|---|---|---|---|
Chemical disinfection | Physical disinfection | |||
杀孢子剂 | 75%乙醇溶液 | 紫外线+臭氧 | ||
Sporicide | 75% alcohol | UV+Ozone | ||
防护用品Protective equipment | ||||
防护服Protective suits | √ | |||
拖鞋 Slippers | √ | √ | ||
口罩Face masks* | √ | √ | ||
帽子Hats* | √ | √ | ||
手套Gloves* | √ | √ | ||
实验用品 Laboratory supplies | ||||
超纯水Ultrapure water | √ | |||
擦拭纸 Wiping paper | √ | |||
离心管Centrifuge tubes | √ | |||
细胞Cell * | √ | |||
细胞计数板Cell counting plates* | √ | √ | ||
细胞培养耗材Cell culture consumables* | √ | √ | ||
细胞培养试剂*Cell culture reagents* | √ | √ | ||
仪器设备Instruments and equipment | ||||
移液器 Pipettors | √ | √ | ||
精密小型仪器Precision small instruments | √ | √ | ||
小型仪器 Small instruments | √ | √ | ||
大型仪器 Large instruments | √ | √ |
Table 3 Various methods of disinfection of laboratory items entering cell culture laboratories of Laboratory Animal Center of Shanghai Jiao Tong University
实验物品 Experimental item | 高压灭菌 Autoclave | 化学消毒 | 物理消毒 | |
---|---|---|---|---|
Chemical disinfection | Physical disinfection | |||
杀孢子剂 | 75%乙醇溶液 | 紫外线+臭氧 | ||
Sporicide | 75% alcohol | UV+Ozone | ||
防护用品Protective equipment | ||||
防护服Protective suits | √ | |||
拖鞋 Slippers | √ | √ | ||
口罩Face masks* | √ | √ | ||
帽子Hats* | √ | √ | ||
手套Gloves* | √ | √ | ||
实验用品 Laboratory supplies | ||||
超纯水Ultrapure water | √ | |||
擦拭纸 Wiping paper | √ | |||
离心管Centrifuge tubes | √ | |||
细胞Cell * | √ | |||
细胞计数板Cell counting plates* | √ | √ | ||
细胞培养耗材Cell culture consumables* | √ | √ | ||
细胞培养试剂*Cell culture reagents* | √ | √ | ||
仪器设备Instruments and equipment | ||||
移液器 Pipettors | √ | √ | ||
精密小型仪器Precision small instruments | √ | √ | ||
小型仪器 Small instruments | √ | √ | ||
大型仪器 Large instruments | √ | √ |
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