Isolation and Identification of Oligodendrocyte Precursor Cells from Tree Shrews Cerebral Cortex

doi:10.3969/j.issn.1674-5817.2019.04.002

Abstract

Abstract: Objective To establish an effective method of isolation, identification and primary culture for oligodendrocyte precursor cells (OPCs) which derived from the cerebral cortex of tree shrews in vitro. Methods Newborn tree shrews within 48 hours were given euthanasia, the cerebral cortex were collected, removed the meninges and brainstem. Then, the cerebral cortex was digested with trypsin and DNase I, and primary cultured. Oligodendrocyte precursor cells were purified by chemical separation and differential adherence. The oligodendrocyte precursor cells were identified by immunofluorescence staining of chondroitin sulfate proteoglycan 4 (NG2) and myelin basic protein (MBP). Results The purified oligodendrocyte precursor cells showed round or oval cell body, with strong refractive and bipolar or tripolar protuberances. The purity of the cells was 97% by immunofluorescence staining of NG2. After adding differentiation medium, oligodendrocyte precursor cells could differentiate and mature, and the immunofluorescence staining of MBP is positive. Conclusion A method for isolation and purification of cerebral cortex oligodendrocyte precursor cells from tree shrews was successfully established.

Key words: Oligodendrocyte precursor cells (OPCs), Cerebral cortex, Primary cell culture

CLC Number:

Q95-33

LI Ming-xue, LI Xiao-hui, HUANG Xin, WANG Xuan, ZHANG Zhi-cheng, YUAN Yuan, LU Cai-xia, SUN Xiao-mei. Isolation and Identification of Oligodendrocyte Precursor Cells from Tree Shrews Cerebral Cortex[J]. Laboratory Animal and Comparative Medicine, 2019, 39(4): 260-266.

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