Induction and Differentiation of Tree Shrew Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells in Vitro

doi:10.3969/j.issn.1674-5817.2017.05.001

Laboratory Animal and Comparative Medicine ›› 2017, Vol. 37 ›› Issue (5): 337-343.DOI: 10.3969/j.issn.1674-5817.2017.05.001

Induction and Differentiation of Tree Shrew Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells in Vitro

MIAO Yu-run, LI Na, KUANG De-xuan, SONG Qing-kai, YUAN Yuan, WANG Xuan, ZHANG Zhi-cheng, LU Cai-xia

Center for Tree shrews Germplasm Resource, Institute of Medical Biology,Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China

Received:2017-03-08 Online:2017-10-25 Published:2017-10-25

Abstract

Abstract: Objective To observe the feasibility of the differentiation of tree shrew (Tupaia belangeri) bone marrow mesenchymal stem cells (BM-MSCs) to neuron-like cells. Method The tree-shrew BM-MSCs were differentiated to neuron-like cells by IMEM differentiation culture mediums (with different inducer and concentration). Identify the secretion concentration of neuron-specific enolase (NES) and α-synuclein(α-SYN) by PCR. NES antibody was also used to identify cell immunofluorescence. Result The result of the tree-shrew BM-MSCs differentiation to neuron-like cells with IMEM cell culture differentiation mediums (10 μg/mL EGF and 20 μg/mL bFGF) were good on 12d in-vitro. Conclusion Optimized culture medium with double grow factors and differentiation method were more appropriated for tree shrew BM-MSCs differentiation to neuron-like cells, and the cells differentiated by this manner were consistent to neuron-like cells characteristics.

Key words: Tree shrew (Tupaia belangeri), Bone marrow mesenchymal stem cells (BM-MSCs)

CLC Number:

Q95-33

MIAO Yu-run, LI Na, KUANG De-xuan, SONG Qing-kai, YUAN Yuan, WANG Xuan, ZHANG Zhi-cheng, LU Cai-xia. Induction and Differentiation of Tree Shrew Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells in Vitro[J]. Laboratory Animal and Comparative Medicine, 2017, 37(5): 337-343.

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Induction and Differentiation of Tree Shrew Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells in Vitro

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