Abstract: The modified method for cultivating an adult buffalo lens epithelial cells was described. The cell growth in vitro and the recovery of cells from frozen stale was investigated by cell counting , MTT and transmission electron microscopy. The results appeared that there is no significant difference in growth between cells cultured in vitro and recovered from frozen stale （P＞0.05），with characteristic and structure similar to the normal epithelial cell. The results suggested that planting culture is a kind of culture method for lens epithelial cells and freezing is an effective method to keep in cryopreservation such cells.

Key words: Lens epithelial cells, Culture, Cryopreservation, Recovery, Ultrastructure