Comparison of Traditional Ultrasound with Speckle Tracking Imaging Technique by Myocardial Infarction Model in Mice

doi:10.3969/j.issn.1674-5817.2018.05.006

Abstract

Abstract: Objective By analyzing and comparing conventional echocardiography (ECG) to speckle tracking imaging (STI) technique on mice myocardial infarction (MI) model, a more precise in vivo monitoring method is able to be demonstrated. Methods The left anterior descending artery (LAD) ligation (MI group) or sham operation (Sham group) were randomly performed in male C57BL/6 mice at 16 weeks age. Same ultrasound parameters were performed on both groups. Followed conventional ECG method to record ejection fraction (EF%) and fraction shortening (FS%) from left ventricle and utilized STI technique to analyze cardiac cycle velocity, displacement, strain and strain rate of each segment in both short axe and long axe. Compared the results with conventional ECG and STI technique. Results Conventional ECG is able to point out MI status in mice, but STI technique can give more information related to myocardial motor ability and mechanics properties. Thus, it's more sensitive and informative than conventional ECG. Conclusion STI technique can be used as a supplementary method for MI monitoring in mice that will improve the precision of evaluation.

Key words: Myocardial infarction (MI), High-frequency ultrasound, Speckle tracking imaging(STI), Strain, Strain rate

CLC Number:

Q95-33

LU Jin, YAN Guo-feng, ZHOU Jin, XIAO Zhong-biao, ZHU Yin-qiu, ZHU Lian, WANG Jing, LI Yao, ZUO Yong. Comparison of Traditional Ultrasound with Speckle Tracking Imaging Technique by Myocardial Infarction Model in Mice[J]. Laboratory Animal and Comparative Medicine, 2018, 38(5): 356-364.

References

[1] 王军,高传玉.心血管疾病动物模型[M].郑州:郑州大学出版社,2014:135-146,162-163.
[2] Parviz Y,Vijayan S,Lavi S.A review of strategies for infarct size reduction during acute myocardial infarction[J].Cardiovasc Revasc Med,2017,18(5):374-383.
[3] Okajima K,Abe Y,Fujimoto K,et al.Comparative study of highresolution microimaging with 30-MHz scanner for evaluating cardiac function in mice[J].J Am Soc Echocardiogr,2007,20(10):1203-1210.
[3] 赵静,曾智,颜亮,等.小动物超声仪与临床用超声仪评价小鼠心脏重构的对比分析[J].南方医科大学学报,2011,31(3):443-447.
[4] 付倩.STI评价大鼠急性心梗模型整体与局部心肌功能改变实验研究[D].武汉:华中科技大学,2010.
[5] Plante E,Lachance D,Roussel E,et al.Impact of anesthesia on echocardiographic evaluation of systolic and diastolic function in rats[J].J Am Soc Echocardiogr,2006,19(12):1520-1525.
[6] Schmidt R,Orwat S,Kempny A,et al.Value of speckle-tracking echocardiography and MRI-based feature tracking analysis in adult patients after Fontan-type palliation[J].Congenit Heart Dis,2014,9(5):397-406.
[7] 王建飞,周艳,刘吉宏,等.实验动物饲养管理和使用指南[M].上海:上海科学技术出版社,2012.
[8] 张芯,马永强,蔡伟,等.小动物超声评价异丙肾上腺素诱导的小鼠心肌缺血[J].中国超声医学杂志,2016,32(2):173-176.
[9] 张鹤萍,钱海超,熊杰,等.阿托伐他汀对异丙肾上腺素诱导的心力衰竭小鼠胞浆内钙火花影响的研究[J].临床和实验医学杂志,2017,16(16):1569-1574.
[10] 吕丹,鲍丹,董伟,等.主动脉缩窄术模型小鼠成模早期评价技术[J].中国实验动物学报,2013,21(4):21-25.
[11] 赵菁,王迎超,张玲,等.超声心动图评价不同心肌梗死模型小鼠心功能的改变[J].中华急诊医学杂志,2015,24(11):1242-1244.
[12] 王琦,魏颖,朱烨,等.小鼠超声心动图成像方法学的探讨[J].中国超声医学杂志,2014,30(20):1120-1122.
[13] 付鹤玲,仝欣,郑媛,等.常用麻醉剂对麻醉小鼠超声心功能的影响[J].现代生物医学进展,2014,14(36):7025-7029.
[14] 黄国倩,潘翠珍,舒先红.心肌应变率成像-超声定量评价局部心肌功能的新技术[J].中国临床医学,2004(5):918-920.
[15] 王金锐,杨颖,杨敬英,等.心肌应变率成像对心梗病人左室心肌局域功能的研究[J].中国医学影像技术,2001,17(9):830-833.
[16] 舒先红,黄国倩,潘翠珍,等.正常人心肌应变及应变率定量分析[J].中华超声影像学杂志,2004,13(11):4-6.
[17] Mondillo S,Galderisi M,Cameli M,et al.Speckle tracking echocardiography-A new technique for assessing myocardial function[J].J Ultrasound Med,2011,30(1):71-83.
[18] 李昊,张幼怡,沈蔷,等.超声二维斑点追踪技术评价小鼠心肌梗死后节段性室壁运动异常[J].中国分子心脏病学杂志,2015,15(3):1354-1358.
[19] Bauer M,Cheng S,Jain M,et al.Echocardiographic speckletracking based strain imaging for rapid cardiovascular phenotyping in mice[J].Circ Res,2011,108(8):908-916.