Abstract: Objective To isolate and identify Helicobacter pylori in Guizhou miniature pig .Methods Specimens of an abnormal growth and development retarded miniature pig was collected.Bacteria isolation and culture，biochemical identification, susceptibility test，serum agglutination test，PCR assay were conducted. Results One strain of Helicobacter pylori (HPp0812) was isolated and identified. The genome of HPp0812 strain could be amplified by 16S rRNA gene of Helicobacter genus and ure A gene of Helicobacter pylori specific primers in PCR assay. Through determination of the nucleotide sequence, the highest sequence homology of this strain of bacteria could be demonstrated with Helicobacter pylori reference strain in GenB epidemiological ank and it was found to be 99%.Conclusion The strain HPp0812 appears to be the Helicobacter pylori first isolated from miniature pig in China, and it provides reliable scientific data for further research and investigation.

Key words: Miniature pig, Helicobacter pylori, Isolation, Identification