Abstract: Objective To observe epithelial sodium channels （ENaC） currents in spermatogenic cells. Method Epithelial sodium channels were obtained in acutely dissociated mouse spermatogenic cells using whole-cell patch clamp. Results The currents were evoked by 200 ms test pulses between-100?+40 mV in 10 mV increments from a holding potential （HP） of -50 mV. Test pulses elicited rapidly activating and non-inactivating Na⁺ current. Na⁺ currents amplitude were significantly reduced by adding Amiloride （1 μmol/L） to the external solution, and displayed two kinds of inhibition effects （the ratio of 58.49%±9.82%，31.44%±5.69%）. Conclusion The characteristics of such currents and sensitive to Amiloride all approved that these currents recorded from mouse spermatogenic cells were produced through epithelial sodium channels.