Abstract: Comparison among ELISA, IEA and HI was made in detecting the antibody to rat Parvovirus.The reproducibilities of ELISA, IEA and HI were 92.5%,88.2% and 68.6% respectively. When the same sera were investigated,the consistencies of the results between ELISA and IEA, HI and ELISA/IEA were 90.5% and 69% respectively,and antibody-positive rates were 81% by both ELISA and IEA, but only 69% by HI. The reproducibilities of the seromonitoring results and the positive rates of antibody by ELISA and IEA were higher than by HI （P＜0.05）, RV antibody was found positive by ELISA in 59% of the conventional rat samples while in clean rat, the positive rate was only 7%.

Key words: ELISA, IEA, HI, Rat, Parvovirus