Table of Content

25 September 1993, Volume 13 Issue 3

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Purification and Analysis of the Circulating Antigens of Schistosomiasis Japonica in Rabbits

Xu Guo-fang1; Peng Min2

1993, 13(3):  125-128. 

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The circulating antigen （CAg） of schistosomiasis japonica from the sera of Schi-stosoma japonicum-infected rabbits was purified by immune-affinity chromatography. The purified CAg exhibited seven protein bands by SDS-PAGE. The molecular weights of them are 144,118, 100，88，63，57 and 46 kD respectively. These CAgs showed five sugar protein bands by PAS colouring. The molecular weights of these sugar proteins are 144,118，100, 63 and 57 kD respectively. The CAgs released from the ova of the schistosome contain 114 and 118 kD sugar proteins as shown by ELIB.

Detection of Anti-Bacillus Piliformis Antibody in Rat Colonies Using Indirect Immunofluorecent Assay

Yao Ju-fang;Tang Jia-ming

1993, 13(3):  129-131. 

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Using Bacillus piliformis extracted from infected liver homogenate as bacterial antigen, an indirect immunofluorecent assay was established for the detection of anti-Bacillus piliformis antibody, and proved to be specific to Bacillus piliformis. By this method, it was found that the antibody positive rate of SPF rats maintained in isolators was 0 （0/28）, while that of conventional rats maintained in open system was 40.9% （34/88），indicating different infective rates of rats in different envi-ronments. The antibody positive rate of conventional mice maintained in the same open system was only 3%.As the bacterial antigen used in this method was derived from rat, whether the difference of antibody positive rate between rats and mice reflects the different antigenicities of the bacteria derived from different species remains for further study.

Comparison among ELISA，IEA and HI for Detection of Rat Parvovirus Antibody

1993, 13(3):  132-134. 

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Comparison among ELISA, IEA and HI was made in detecting the antibody to rat Parvovirus.The reproducibilities of ELISA, IEA and HI were 92.5%,88.2% and 68.6% respectively. When the same sera were investigated,the consistencies of the results between ELISA and IEA, HI and ELISA/IEA were 90.5% and 69% respectively,and antibody-positive rates were 81% by both ELISA and IEA, but only 69% by HI. The reproducibilities of the seromonitoring results and the positive rates of antibody by ELISA and IEA were higher than by HI （P＜0.05）, RV antibody was found positive by ELISA in 59% of the conventional rat samples while in clean rat, the positive rate was only 7%.

Analysis of the Somatic and Capsular Antigens of Pasteurella Multocida

Shen;Xin-liang;Yuan;Hao-jia;Wei;Tian-wen;Ning;Lei;Chen;Hong;Wu;Xiang-lin

1993, 13(3):  135-139. 

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Crude somatic and capsular antigens were prepared respectively from Pasteurella multocida P-1059 strain （serotype 3） and A-898 strain （capsular group A） by heat-extraction. The crude extracts（CE） were purified by ammonium sulfate precipitation or high-pressure liquid chromatography （HPLC, G 4000）. ELISA, SDS-PAGE and enzyme treatment were used to analyse the biological properties of the purified materials. The results were as follows：（1） Binding activity of the precipitate to antibody rose with the increase of ammonium sulfate concentration.（2） Four protein peaks of P-1059 and three peaks of A-898 were obtained by HPLC. Antigenic reac-tivity was detectable only in the first peak of the both strains. （3）Upon SDS-PAGE, peak 1 of P-1059 showed three protein bands, corresponding to molecular wei -ghts of 54300, 45900 and 38300. （4）Purified materials of P-1059 and A-898 were treated respectively by pronase E, proteinase K,neuramidinase and sodium periodate. After treated with sodium periodate, the two kinds of antigen were not reactive to their specific antisera.It suggested that the antigenic determinant may be relative to carbohydrate. （5）The result of cross inhibition by ELISA between the purified materials of P-1059 and A-898 indicated that the former contained trace ingredient of the latter.