Effect of 60Co γ-ray Radiation on Spleen of Naked Mole Rats

doi:10.3969/j.issn.1674-5817.2020.06.009

Abstract

Abstract: Objective To observe the changes of immune system and tissue biochemical indexes in the spleen of naked mole rats exposed to ⁶⁰Co γ-ray radiation, for probing the radiation tolerance characteristics of the spleen of naked mole rats. Methods The whole body of naked mole rats was irradiated with a radiation dose rate of 1.163 Gy/min and a total dose of 10 Gy ⁶⁰Co γ-rays. The spleens of the normal control and the exposed naked mole rats after 7 d, 14 d, and 21 d of irradiation were collected. The proportions of B lymphocytes and macrophages in the spleens were measured by flow cytometry. Malondialdehyde (MDA), total antioxidative capacity (T-AOC), glutathione (GSH) and oxidative glutathione (GSSG) in the spleen tissues were measured with corresponding biochemical assay kits. Results Flow cytometry results showed that the numbers of both B lymphocyte and macrophages increased significantly after irradiation (P<0.001). Biochemical examination results showed that the MDA level in spleen was significantly increased (P<0.05) after irradiation, the GSH and GSSH levels were significantly decreased (P<0.05), the T-AOC level was first significantly decreased (P<0.01) and then raised 21 days after irradiation, which was significantly higher than the indexes 7 days and 14 days after irradiation (P<0.01). Conclusion The spleens of naked mole rats can tolerate ⁶⁰Co γ-ray radiation to some extent, but the mechanism of tolerance is not clear.

Key words: Naked mole rats, Radiation tolerance, Spleen, Immunology, ⁶⁰Co ?-rays

CLC Number:

Q95-33

YANG Rong, ZHAO Yining, YANG Wenjing, ZHAO Shanming, CHAI Yujing, ZHANG Chengcai, YUAN Zheng, CUI Shufang. Effect of ⁶⁰Co γ-ray Radiation on Spleen of Naked Mole Rats[J]. Laboratory Animal and Comparative Medicine, 2020, 40(6): 519-522.

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Effect of ⁶⁰Co γ-ray Radiation on Spleen of Naked Mole Rats

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