Investigation on Radiosensitivity Impact of Lung Cancer A549 Cells by Vascular Endothelial Growth Factor Silencing in vivo

doi:10.3969/j.issn.1674-5817.2019.04.005

Abstract

Abstract: Objective To research the radiosensitivity of A549 cells in tumor-bearing mouse model by knock down vascular endothelial growth factor (VEGF) gene. Methods The tumor-bearing mouse model was established by using A549 cells which was cultured in vitro. The siVEGF/RNAi-MateTM mixture was injected into the tumor of model mice and with radiotherapy. The nude mice were euthanasia on day 24 after radiotherapy. The expressions of VEGF were detected by immuno-histochemical, Q-PCR and Western blotting. Results Both the protein and mRNA levels of VEGF in VEGF knockdown and radiotherapy group were significantly decreased compared with the control group (P<0.05). The volume of tumor was decreased and the necrotic area of tumor were increased distinctly in siVEGF transfected and radiotherapy group compared with control group (P<0.05). Conclusion Knock down of VEGF and radiotherapy can inhibit tumor growth, and increase the radiosensitivity of lung cancer radiotherapy.

Key words: Lung cancer, A549, siRNA, Vascular endothelial growth factor (VEGF), Radiosensitivity

CLC Number:

Q95-33

MA Jun, LUO Jun-dong, JING Wen-jiang, ZHANG Shu-lian, WANG Juan-yi, FAN Zhi-gang. Investigation on Radiosensitivity Impact of Lung Cancer A549 Cells by Vascular Endothelial Growth Factor Silencing in vivo[J]. Laboratory Animal and Comparative Medicine, 2019, 39(4): 280-286.

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