The Role of MCP-1, CD68 and ICAM-1 in the Process of Angiogenesis in Choke Area of Mice

doi:10.3969/j.issn.1674-5817.2017.06.002

Abstract

Abstract: Objective To explore the role of inflammation in angiogenesis in choke area. Methods The ear flap model of mice was established and the changes of blood vessel diameter was observed by HE staining. The expressions of intercellular cell adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein-1 (MCP-1) and cluster of differentiation 68 (CD68 ) were detected by immunohistochemistry on the day after modeling(0 d), 1 d, 3 d, 5 d and 7 d, respectively. Result By observing the vascular diameter of the ear flap in mice, it was found that the vessels were visible at 3 d after operation and the vascular dilatation was obvious at 7 d. CD68-labeled macrophages had the largest accumulation in two choke area at 3 d. High expression of MCP-1 and ICAM-1 was observed in the early stage of postoperative inflammatory response. The expression of the three inflammatory factors in the tissue was increased significantly during the vascular dilation. Conclusion The three inflammatory factors (ICAM-1, CD68 and MCP-1) play an important role in vascular remodeling. Proper inflammatory response plays a positive role in flap survival.

Key words: Immunohistochemistry, Inflammatory factor, Choke area, Vessels

CLC Number:

Q95-33

WEN Fu-li, LIANG Cheng, ZHUANG Yue-hong, Ma Lei, ZHENG He-ping. The Role of MCP-1, CD68 and ICAM-1 in the Process of Angiogenesis in Choke Area of Mice[J]. Laboratory Animal and Comparative Medicine, 2017, 37(6): 428-433.

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