Laboratory Animal and Comparative Medicine ›› 2018, Vol. 38 ›› Issue (4): 261-266.DOI: 10.3969/j.issn.1674-5817.2018.04.004

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Comparative Analysis on Rat Models of Parkinson's Disease Established by Injecting 6- hydroxy Dopamine with Different Sites

LI Min1, CHANG Xue-hui2, ZHANG Liang-zhi2, LEI Zhen3, LUO Shen1   

  1. 1. Zhoukou Hospital of Traditional Chinese Medicine,Henan,Zhoukou 466000,China;
    2. Henan University of CM,Zhengzhou 450002,China;
    3. Central Laboratory of Henan Province Hospital of TCM,Zhengzhou 450002,China
  • Received:2018-01-14 Online:2018-08-25 Published:2021-03-01

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Abstract: Objective To explore the way of establishment of stable and effective Parkinson's Disease(PD) rats model with 6-hydroxyl dopamine (6-OHDA).Methods Firstly,SD rats were randomly divided into four groups,the one spot via midbrain group,the double spots via midbrain group,the one spot-twice injections via striatum group and control group.And 6-OHDA was injected into the brain of rats respectively according to different groups.Then observed the behavioral changes of rats respectively in the first week,second week,fourth week and eighth week,and tissue of brain was obtained after observation of behavioral changes in the eighth week,and then pathological examination was performed by HE staining and immunohistochemistry.Finally,tyrosine hydroxylase (TH) positive cells were analyzed with biosens digital imaging system.Results ① The success rate of PD rats of the one spot via midbrain group was 72.7%,the double spots via midbrain group was 60%,the one spot-twice injections via striatum group was 47.6%,and control group was 0%.The success rates of different groups were statistically significant (P<0.05).②The average revolving number of successful PD model of the one spot via midbrain group in the first week was 7.3±0.2 r/min,and increased than the first week in the second week (P<0.05),increased than the second week in the fourth week (P<0.05),and increased than the fourth week in the eighth week,but there was no statistically significant (P>0.05).There was the similar behavioral change trend in the double spots via midbrain group and the one spot-twice injections via striatum group,but there was no statistical significance at the same time among different groups (P>0.05).③ After analysis of TH positive cells of the damaged side of PD rats with biosens digital imaging system,the average integral optical density (IOD) could be obtained,and the IOD of the one spot via midbrain group,the double spots via midbrain group and the one spot-twice injections via striatum group was significantly smaller than that of the control group (P<0.01),and the IOD of the one spot via midbrain group was smaller than that of the double spots via midbrain group and the one spot-twice injections via striatum group (P<0.05).Conclusions The results suggest that after injection of 6-OHDA into the substantia nigra pars compact singly,the success rate of PD rats is higher,the PD rats is more stable and the mortality rate is lower.

Key words: Parkinson's Disease(PD), Rat model, 6-hydroxyl dopamine (6-OHDA), Immunohistochemistry

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