Study on Gut Hormones in GK Rats after Different Length Duodenum-Preserving Duodenal-Jejunal Bypass Surgery

doi:10.3969/j.issn.1674-5817.2014.03.011

Abstract

Abstract: Objective To establish the different length duodenum-preserving duodenal-jejunal bypass model in GK rats and explore the changes in gut hormones after surgery. Methods Male Goto-kakizaki diabetic rats(GK, n=24) were randomly divided into four groups: group I (sham group), group II(regular DJB surgery), group III (8mm duodenum-preserving DJB surgery), group IV(18mm duodenum-preserving DJB surgery). Carotid artery intubation was performed ninth week after surgery. OGTT was performed one week after carotid artery intubation . The change of the level of the serum index (blood glucose, insulin, GLP-1, GIP) during OGTT was measured. Results All GK rats after surgery were with a long-term survival. The OGTT, first-phase insulin and GLP-1 secretion in group III were improved significantly compared with group I (P<0.05) and the initial secretion of GIP in group III was significantly fewer than group I (P<0.05). The OGTT, first-phase insulin and GLP-1 secretion , the initial secretion of GIP in group IV had no significant difference compared with group I (P>0.05). The first-phase insulin and GLP-1 secretion in group II were significantly higher than group IV (P<0.05) and the GIP secretion at 30 min was significantly higher than group IV (P<0.05). Conclusions On the basis of experimental results, the mechanisms of DJB surgery improving blood glucose level in GK rat might be closely related to the restoration of balance between anti-incretin factors and incretin factors. On the other hand, DJB surgery can improve insulin-resistance in GK rat through reducing the secretion of GIP.

Key words: Type 2 diabetes mellitus, Goto-kakizaki rat, Duodenal-jejunal bypass surgery, Duodenum-preserving, Glucagon-like peptide-1, Glucose-dependent insulinotropic polypeptide

CLC Number:

Q95-33

CHEN Li-Xin, ZHAO Xian-zhe, QIAO Wei-wei, GU Jian-Zhong, WANG Long-Xia. Study on Gut Hormones in GK Rats after Different Length Duodenum-Preserving Duodenal-Jejunal Bypass Surgery[J]. Laboratory Animal and Comparative Medicine, 2014, 34(3): 223-227.

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