Investigation on Helicobacter spp. Infection in Rat, Mouse, Guinea Pig and Rabbit

doi:10.3969/j.issn.1674-5817.2015.02.007

Abstract

Abstract: Objective To survey the Helicobacter spp. among rats, mice, guinea pigs and rabbits in Shanghai and surrounding area. Method The ileocecal region contents of rats, mice, guinea pigs and feces of rabbits were collected, and the bacterial genomic DNA were extracted. Both traditional PCR and multiplex PCR test were performed to investigate the Helicobacter spp. infection. Result The positive rate of Helicobacter spp. in rat, mouse, guinea pig and rabbit were 50.5%(105/208), 37.9%(204/538), 36.7%(36/98) and 4.9%(12/247) respectively. In addition, the triplex PCR test results of H.hepaticus, H.bilis, H.rodentium showed that H.hepaticus, H.bilis, infection can be detected in rabbit. H.bilis, H.rodentium can be detected in guinea pig. H.hepaticus, H.bilis, H.rodentium can be detected in rat and mouse. Conclusions There are different degrees of Helicobacter spp. infection in rats, mice, guinea pigs and rabbits of Shanghai and surrounding area.

Key words: Helicobacter spp., PCR, Rodent, Rabbit

CLC Number:

Q95-33

FENG Jie, ZHANG Rong, XIE Jian-yun, LI Rui-jiao, HU Jian-hua, GAO Cheng. Investigation on Helicobacter spp. Infection in Rat, Mouse, Guinea Pig and Rabbit[J]. Laboratory Animal and Comparative Medicine, 2015, 35(2): 120-124.

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