Breeding and Identification of BAMBI Gene Knockout Mice

doi:10.3969/j.issn.1674-5817.2014.05.007

Abstract

Abstract: Objective Breed and identify BAMBI knockout mice for studying the role of BAMBI in obesity and energy metabolism. Methods The introduced BAMBI^-/- mice were paired ,and the progenies were back-crossed to their wild type mice (C57BL/6J background). The offspring were inter-crossed to obtain sufficient number of the knockout mice, the genome DNA was extracted from the tail of the mice for genotyping by PCR. The BAMBI mRNA expression in liver, brown adipose tissue, subcutaneous and gonadal white adipose tissue of BAMBI knockout and wild type mice were detected by real time PCR. Results The inter-crossing results of heterozygous mice with BAMBI gene were basically accordant with Mendelian inheritance laws. The BAMBI mRNA expression was hardly detected in BAMBI knockout mice. Conclusion It is feasible to breed BAMBI knockout mice by inter-crossing of the heterozygote. PCR technique can be used to identify the genotype of BAMBI knockout mice precisely.

Key words: BAMBI, Knockout, Genotyping, PCR, Real time PCR

CLC Number:

Q95-33

LUO Xiao, WEI Shuang-yu, JIA Ru, YAO Ting, ZHANG Yan-ru, SHI Xiao-bo, YAN Jian-qun. Breeding and Identification of BAMBI Gene Knockout Mice[J]. Laboratory Animal and Comparative Medicine, 2014, 34(5): 381-385.

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