The Effect of Curcumin Pretreatment on Flora Expression of Ileum and Colon Contents in Heatstroke Rats with Simulated Desert Environment

doi:10.3969/j.issn.1674-5817.2018.06.007

Abstract

Abstract: Objective To investigate the effect of Curcumin on the flora expression of ileum and colon content in heatstroke rats with simulated dry heat desert environment. Methods Fifty male SD rats were randomly divided into 5 groups, with 10 rats in each group, including normal temperature and humidity group (control group), mild heat stroke group, moderate heat stroke group, severe heat stroke group, and severe heat stroke with Curcumin intervention group. The rats in Curcumin intervention group were intragastrically administrated with 200 mg/kg Curcumin suspension (1 mL/100 g body weight) for 7 successive days and the rest of the groups were continuously irrigated with saline for 7 days. Different degree of heat stroke rat models were established with the northwest special artificial environment laboratory chamber in our laboratory as previous ways. Animals were anesthetized and sterile specimens of ileum and colon contents were frozen in liquid nitrogen, PCR was used to analyze the expression of Escherichia coli, Enterococcus, Lactobacillus, Bacillus bifidus in the rats of each group. Results Heatstroke rats in simulated dry heat desert environment, the Escherichia coli and Enterococcus expression in their ileum and colon were increased significantly (P<0.05), the expression of Lactobacillus did not change significantly, and Bifidobacterium expression was significantly decreased (P<0.01). After intervention with Curcumin, Escherichia coli, Enterococcius expression was significantly decreased (P<0.05), the expression of Lactobacillus did not change significantly, but Bifidobacterium expression was significantly increased (P<0.01). Conclusion Curcumin may improve the expression of bacteria Bifidobacterium in the ileum and colon of heatstroke rats, inhibit opportunistic pathogenic Escherichia coli and enterococci proliferation in ileum and colon, therefore, keep the intestinal flora in homeostasis, reduce the degree of intestinal infection and enhance the rats' tolerance to heatstroke.

Key words: Curcumin, Heat stroke, Ileum, Colon, Intestinal bacteria

CLC Number:

Q95-33

XU Qin, DONG Xiang, LI Jian-ying, ZHANG Dong-hui, LI Jia-jia, YANG Ya-ping, YANG Qiu-yue, SHI Wen-hui, MA Na, SONG Lai-yang, LIU Jiang-wei. The Effect of Curcumin Pretreatment on Flora Expression of Ileum and Colon Contents in Heatstroke Rats with Simulated Desert Environment[J]. Laboratory Animal and Comparative Medicine, 2018, 38(6): 440-445.

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