Anatomical Characteristics and Background Lesions in Laboratory British-shorthair Cats

doi:10.12300/j.issn.1674-5817.2021.151

Abstract

Abstract:

Objective To investigate anatomical characteristics and background lesions in laboratory British-shorthair cats (BSCs) through gross and histopathological examination, and provide effective basis for the research and application, standard establishment and market promotion of this experimental animal. Methods Gross system dissection and histopathological examination were performed on 14 adult BSCs (half male and half female), and the histological characteristics and background pathological data were collected and analyzed. Results The body weight and organ index of spleen of BSCs was higher in males than females (P < 0.05, P < 0.01), the organ coefficients of brain and thymus in BSCs were lower in males than females (P < 0.05). The anatomical structure of testicles, heart, thyroid gland and tentorium of BSCs is different from that of rodents and canine. Furthermore, histopathological examinations revealed that the structures of heart, pancreas, spleen in BSCs differed from two experimental animals mentioned above. In addition, there were multiple hepatic cell vacuolar degeneration in BSCs (9/14), and occasional renal, brain and lymphoid tissues were also found in some animals. Conclusion There were six differences in body weight, and organ-coefficient of spleen, thymus, and brain in BSCs, while the hepatocyte phospholipidosis of liver and background lesion in BSCs were found.

Key words: British-shorthair Cat, Gross autopsy, Histopathological examination, Anatomic characteristic, Background lesion

CLC Number:

Q95-33

Yu WANG, Dan GAO, Nana PENG, Ai HANG, Bin SHEN, Jian HUANG, Liming TANG. Anatomical Characteristics and Background Lesions in Laboratory British-shorthair Cats[J]. Laboratory Animal and Comparative Medicine, 2022, 42(3): 229-236.

Figures/Tables 4

Figure 1 The general appearance （A）， facial view （B）， abdominal cavity （C）， thoracic cavity （D） and cranial cavity （E） of laboratory British-shorthair cats

Table 1 Organ index of male and female laboratory British-shorthair cats

	雄性Male		雌性 Female
脏器 Organ	脏器平均质量 Organ weight/g	脏器指数 Organ index/%	脏器平均质量 Organ weight/g	脏器指数 Organ index/%
心脏Heart	17.72 ± 1.92	0.46 ± 0.09	10.35 ± 2.36	0.40 ± 0.02
肝脏Liver	103.34 ± 9.79	2.70 ± 0.50	69.32 ± 118.19	2.68 ± 0.57
脾脏Spleen	6.41 ± 0.46	0.71 ± 0.10	4.53 ± 1.16	0.18 ± 0.05^**
肺脏Lung	21.14 ± 1.61	0.55 ± 0.10	15.59 ± 1.36	0.61 ± 0.04
肾脏Kidney	38.30 ± 1.35	1.00 ± 0.15	23.72 ± 5.82	0.91 ± 0.08
脑Brain	27.40 ± 0.82	0.71 ± 0.10	26.71 ± 0.23	1.05 ± 0.13^*
胸腺Thymus	1.23 ± 0.43	0.03 ± 0.01	1.23 ± 0.43	0.05 ± 0.02^*
肾上腺Adrenal	0.65 ± 0.05	0.02 ± 0.00	0.71 ± 0.12	0.03 ± 0.01
甲状腺Thyroid	0.38 ± 0.01	0.01 ± 0.00	0.38 ± 0.03	0.01 ± 0.00
睾丸Testis	3.71 ± 0.28	0.10 ± 0.02	-	-
卵巢Ovary	-	-	0.72 ± 0.08	0.03 ± 0.00
子宫Uterus	-	-	3.50 ± 0.33	0.14 ± 0.02

Figure 2 The histopathological characteristics of the main tissues and organs of the laboratory British-shorthair cats detected by HE stainingNote： A is heart， B is aorta， C is spleen， D is pancreas， E is adrenal， F is stomach， G is jejunum， H is ileum， I is cecum， J is liver， K is lung， L is kidney， M is brain， N is testis， O is ovary， P is sternum. The scale of G is 1 000 μm， and the other images scale are 200 μm.

Figure 3 Background lesions of laboratory British-shorthair catsNote： Histopathological characteristics in the heart （A）， kidney （B and C）， liver （D）， lymphonodi cervicales （E）， cerebellum （F）， brainstem （G）， adrenal （H） and lung （I） of laboratory British-shorthair cats stained with H & E （Scale=200 μm）. Arrows in A， B， D， F and G show the vacuolation （fatty） degeneration； arrows in C show the damaged tubular of kidney； arrows in E show the passive congestion of lymph sinus； arrows in H show the disorder structure of the zona fasciculata in the adrenal gland； arrows in I show the bronchus abscess in the lung.

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