环介导等温扩增技术及其应用

doi:10.3969/j.issn.1674-5817.2014.02.018

摘要/Abstract

摘要： 环介导等温扩增法(LAMP)是一种新的核酸扩增法。该方法在一定温度下一步即可完成扩增反应。由于其扩增效率极高、反应简单快速、高特异性和反应结果只需肉眼观察的特点,LAMP法已广泛应用于病毒、细菌等的定性定量检测和临床疾病的诊断。本文介绍了LAMP方法的原理及其在微生物分子检测方面的应用。

关键词: 环介导等温扩增法(LAMP), 核酸扩增, 基因检测

Abstract: Loop-mediated isothermal amplification(LAMP) is a novel nucleic acid amplification method. At a certain temperature, LAMP performed only one step to terminate the process. Due to its high efficiency, simplicity, rapidly, high specificity and the result of LAMP is easily detected by naked eyes, LAMP has been widely applied in the qualitative and quantitative detection of viruses, bacteria and clinical diagnosis of disease. In this paper, we introduced the principle of LAMP and its applicability in molecular detection and diagnosis.

Key words: Loop-mediated isothermal amplification(LAMP), Nucleic acid amplification, Gene detection

中图分类号:

Q95-33

赵丽娟, 周洁, 高诚. 环介导等温扩增技术及其应用[J]. 实验动物与比较医学, 2014, 34(2): 162-166.

ZHAO Lijuan, ZHOU Jie, GAO Cheng. Loop-mediated Isothermal Amplification Technique and Its Application[J]. Laboratory Animal and Comparative Medicine, 2014, 34(2): 162-166.

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