慢性束缚应激诱导自发性高血压大鼠出现抑郁焦虑样行为

doi:10.3969/j.issn.1674-5817.2014.02.003

实验动物与比较医学 ›› 2014, Vol. 34 ›› Issue (2): 93-97.DOI: 10.3969/j.issn.1674-5817.2014.02.003

慢性束缚应激诱导自发性高血压大鼠出现抑郁焦虑样行为

曹爽¹, 丁建伟², 李炳³, 袁丽粉¹, 杜勤¹

1.上海交通大学医学院附属第九人民医院, 上海 200011;
2.云南中医学院针灸推拿康复学院, 昆明 650500;
3.复旦大学附属金山医院, 上海 201508

收稿日期:2013-10-12 出版日期:2014-04-25 发布日期:2014-04-25
作者简介:曹爽(1988-), 女, 硕士研究生, 从事老年高血压与抑郁症的研究。E-mail: caoshuang2209@163.com
基金资助:
上海市科委资助项目(074119621)

Chronic Restraint Stress Induced Anxiety- and Depression-like Behaviors in Spontaneously Hypertensive Rats

CAO Shuang¹, DING Jian-wei², LI Bing³, YUAN Li-fen¹, DU Qin¹

1. Shanghai Ninth People's Hospital Affiliated Shanghai Jiao Tong University School of Medicine, Shanghai 20001, China;
2. School of Acupuncture, Massage and Rehabilitation, Yunnan College of Traditional Chinese Medicine, Kunming 650500, China;
3. Affiliated Jinshan Hospital, Fudan University, Shanghai 201508, China

Received:2013-10-12 Online:2014-04-25 Published:2014-04-25

摘要/Abstract

摘要： 目的探讨自发性高血压大鼠(SHR)在慢性束缚应激(Chronic Restraint Stress, CRS)时抑郁焦虑样行为学改变。方法雄性SHR大鼠16只,随机分为对照组(CON, n=8)和慢性束缚应激组(CRS, n=8)。通过旷场试验(Open Field test, OFT)、强迫游泳实验(Forced Swimming test, FST)、高架十字迷宫试验(Elevated Plus Maze, EPM)评价其抑郁焦虑样行为,同时比较SHR血压变化及血清ACTH水平。结果束缚4周后,与对照组相比,CRS组的SHR在OFT中直立次数及总位移均显著降低(P<0.05),FST中不动时间显著延长(P<0.05),EPM中开臂滞留时间百分比显著下降(P<0.05)。结论慢性束缚应激可诱导SHR出现抑郁焦虑样行为。

关键词: 自发性高血压大鼠(SHR), 慢性束缚应激(CRS), 抑郁, 焦虑

Abstract: Objective To investigate the effects of chronic restraint stress (CRS) on anxiety- and depression-like behaviors in Spontaneously Hypertensive Rats(SHR). Methods SHR were randomly divided into control(CON) group and CRS group respectively. The changes of behaviors were detected by open field test (OFT),forced swimming test (FST) and elevated plus maze(EPM) testŁ¬the changes of blood pressure and levels of adrenocorticotropic hormone(ACTH) in plasma in different groups were also measured and compared. Results SHR in CRS group showed significant reductions in rearing incidence in OFT(PŁĽ0.05),the time spent in open arms during the EPM(PŁĽ0.05), and the immobility during the FST was increased significantly (PŁĽ0.05). Furthermore, the blood pressure and concentration of plasma ACTH increased significantly in CRS group compared to CON group. Conclusions CRS can induce anxiety- and depression-like behaviors in SHR.

Key words: Spontaneously Hypertensive Rat(SHR), Chronic Restraint Stress(CRS), Depression, Anxiety

中图分类号:

Q95-33

曹爽, 丁建伟, 李炳, 袁丽粉, 杜勤. 慢性束缚应激诱导自发性高血压大鼠出现抑郁焦虑样行为[J]. 实验动物与比较医学, 2014, 34(2): 93-97.

CAO Shuang, DING Jian-wei, LI Bing, YUAN Li-fen, DU Qin. Chronic Restraint Stress Induced Anxiety- and Depression-like Behaviors in Spontaneously Hypertensive Rats[J]. Laboratory Animal and Comparative Medicine, 2014, 34(2): 93-97.

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慢性束缚应激诱导自发性高血压大鼠出现抑郁焦虑样行为

Chronic Restraint Stress Induced Anxiety- and Depression-like Behaviors in Spontaneously Hypertensive Rats

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