实验动物与比较医学 ›› 2018, Vol. 38 ›› Issue (6): 446-449.DOI: 10.3969/j.issn.1674-5817.2018.06.008

• 论著 • 上一篇    下一篇

小鼠指甲再生模型建立方法的探索

张新艳1, 关玉婷2, 李大力2   

  1. 1.复旦大学附属妇产科医院研究所,上海 200011;
    2.华东师范大学生命科学学院,上海 200241
  • 收稿日期:2018-08-27 出版日期:2018-12-25 发布日期:2021-03-01
  • 作者简介:张新艳(1989-),女,硕士,初级技师,研究方向:干细胞。E-mail:1989117xyz@sina.com
  • 基金资助:
    国家自然科学基金项目(No.313714552014-2017)

Probing on Establishment of Nail Regeneration Model in Mouse

ZHANG Xin-yan1, GUAN Yu-ting2, LI Da-li2   

  1. 1. Hospital and Institute of Obstetrics and Gynecology Affiliated to Fudan University,Shanghai 200011,China;
    2. School of Life Science,East China Normal University,Shanghai 200241,China
  • Received:2018-08-27 Online:2018-12-25 Published:2021-03-01

摘要: 目的 为研究潜在功能基因在小鼠指甲生长过程中的作用,在国外研究基础上建立并优化指甲干细胞的小鼠指甲再生模型。方法 以8周龄C57BL/6小鼠为实验对象,通过麻醉后在显微镜下精准位置截肢处理进行动物造模、通过实时观察记录、取样测量、切片染色等一系列研究方法的探索与实践,对研究步骤进行适当优化。结果 形成了系统的小鼠指甲再生模型研究手段。结论 该模型能够在动物整体水平、组织水平对小鼠指甲生长情况进行评估,可以成为研究指甲干细胞及相关功能基因的有效手段。

关键词: 指甲再生, 长度测量, HE染色

Abstract: Objective To study the role of potential functional genes in the growth of mouse nails, and to establish and optimize mouse nail regeneration model of nail stem cells based on foreign research. Methods The 8-week-old C57BL/6 mice were selected as the experimental object, and precise position amputation surgery was performed after anesthesia under the microscope. The exploration and practice of a series of research methods such as animal modeling, real-time observation and recording, sampling measurement and section staining were carried out, and the research procedures were optimized as appropriate. Results A systematic study method for mouse nail regeneration animal model was developed. Conclusion This model can evaluate the growth of mouse nails at the animal level and tissue level, and it can be an effective means to study nail stem cells and related functional genes.

Key words: Nail regeneration, Length measurement, HE staining

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