关键词: 细胞培养, 氧化损伤, 细胞模型

Abstract: Objective To explore the best concentration of HzOz to induce hippocampal cellular oxidative damage in rats. Method The hippocampal cell from the rat was co-cultured with H₂O₂ in diversity concentration for 24 hours. The changes of the cell morphology was observed, the viability of hippocampal neures was detected with MTT method; the expression of Bax and Bcl-2 were detected by immunohistochemical method. Result Compared with normal group, when the concentration of H₂O₂ is higher than lmmol/L,the morphology damage of hippocampal cell is obviously aggravated, the cytoactive is low,the Bax is high expression. When the concentration of H₂O₂ is lower than lmmol/L, the morphology damage of hippocampal cellular is not obviously aggravated, the cytoactive has no obvious change, the Bcl-2 is high expression. Conclusion The best concentration of H₂O₂ to establish cell oxidative damage model is lmmol/L.

Key words: Cell culture, Oxidative damage, Cell model