实验动物与比较医学 ›› 2008, Vol. 28 ›› Issue (2): 118-120.

• 论文 •    

体外受精对小鼠早期生长发育的影响及性成熟期血清生化指标的分析

  

  1. 1.复旦大学，上海 200032；2.上海实验动物研究中心，上海 200032；3.上海市计划生育科学研究所，上海 200032；4.南京农业大学农业部动物生理生化重点开放实验室，南京 210095
  • 基金资助:
    上海市科技发展基金（No.054909001）

Effect of in vitro Fertilization on Early-embryo Development and Serum Biochemistry in Sexual Maturity Mouse

  1. 1. Fudan University, Shanghai 200032, China; 2. Shanghai Laboratory Animal Research Center Shanghai 200032,China; 3. Shanghai Institute of Planned Parenthood Research, Shanghai 200032,China; 4. Key Laboratory of Animal Physiology& Biochemistry, Nanjing Agricultural University, Nanjing 210095，China

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摘要： 目的 研究体外受精技术对小鼠早期生长发育的影响，了解性成熟期小鼠的生理状况.方法 将体外受精的2-细胞胚胎植入假孕母鼠输卵管，待胚胎发育至 14 d胚龄，手术取出，称胚胎重； 另有部分母鼠自然产下仔鼠，称取仔鼠的出生重； 待其成长至性成熟期（6周龄），取血测其血清生化指标.结果 体外受精小鼠14 d胚胎重比对照组高16.5%（P＜0.001）； 仔鼠初生重比对照组高13.2%（P＜0.001）； 而性成熟期小鼠的血清生化值与对照组无明显差异.结论 体外受精影响小鼠早期胚胎发育，但不影响性成熟期小鼠的血清生化值.

Abstract: Objective To evaluate the effect of in vitro fertilization (IVF) on early embryo development and serum biochemistry in sexual maturity mouse. Methods The 2-cell embryos from IVF were transferred into pseudo-pregnant recipient mice. The fetus were weighed afler the embryo developed to 14 days (El4).Meanwhile，several pregnant mice were maintained until the pups were bom and weighed. The newborns were raised until sexual maturity, and the serum biochemistry of the mice was analyzed. Result The weight of E14 fetus from IVF group was 16.5% higher than that of the control group (P＜0.001)，the birth weight of IVF group was 13.2% higher than that of control group (P＜0.001)，but the differences of serum biochemistry in sexual maturity mouse between IVF group and control group were not significant. Conclusion The in vitro fertilization can affect the mouse early-embryo development, but it doesn’t affect the serum biochemistry in sexual maturity mouse.

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