气相液氮运输罐短期储存胚胎的可行性研究

›› 2008, Vol. 28 ›› Issue (1): 18-22.

所属专题：实验动物设施设备

气相液氮运输罐短期储存胚胎的可行性研究

1.上海斯莱克实验动物有限公司，上海 201615；2.沈阳农业大学畜牧兽医学院，沈阳 110161

收稿日期:2007-08-22 出版日期:2008-01-31 发布日期:2008-01-31
基金资助:
国家十一五科技支撑项目（2006BA123B00）

Saving Mice Embryos in Short Time by Using Vapour Nitrogen Jar

1. Shanghai SLACCAS lab animal com.limited, Shanghai 201615, China;2. Farming and Veterinary College, Shenyang Agriculture University, Shengyang 110161, China

Received:2007-08-22 Online:2008-01-31 Published:2008-01-31

摘要/Abstract

摘要： 目的探讨用气相液氮运输罐运输小鼠胚胎和短时间保存小鼠胚胎的可行性.方法实验使用 4 个小鼠品系（C57BL/6、B6D2、DBA/2、B10A），通过体外授精的方法得到2-细胞胚胎，用玻璃化冷冻法冷冻胚胎并储存于液相液氮罐，然后转移至气相液氮运输罐内放置14 d再转入液相液氮罐，最后进行复苏移植.结果 4 个品系的冷冻胚胎的复苏率分别为 69.7%、77.27%、77.59%、72.64%，对照组的相应复苏率为97%、84.38%、75%、71.43%，差异均不明显（P＜0.05）.4 个品系胚胎移植后，除 B10A 这个品系的出生率差异显著外（P＜0.01），其他 3 个品系的差异均不显著（P＞0.05）.结论在气相液氮运输罐短时间内储存胚胎或通过气相液氮运输罐进行胚胎运输是可行的.

关键词: 小鼠胚胎, 气相液氮运输罐, 低温保存

Abstract: Object Studing the feasibility of saving mice embryos in vapour nitrogen transport jar in short time. Method we used in vitro fertilization to get the 2-cell embryos of C57BL/6J, B6D2F1, DBA/2, B10A，these embryos were vitrificated in liquid nitrogen，then transferred to vapour nitrogen jar. About 14 days later，these embryos were transferred to liquid nitrogen again for some time. Last, these embryos were post-thawed and transferred to psudopregnant mice. Result The rate of the post-thawmg of the embryos from C57BL/6J，B6D2F1, DBA/2, B10A were 69.70%, 77.27%, 77.59%, 72.64%, and the control groups (embryos vitrificated in liquid nitrogen only) were 97.00%、84.38%、 75.00%、71.43% respectively, there was no difference in the two groups (P＜0.05). When transferred to psudopregnant, there were no difference in C57BL/6J, B6D2F1, DBA/2 in tne rate of birth. But for B10A，the difference was significant(P＜0.01). Conclution The vapour nitrogen transport jar is suitable to cryopreserve and transport mice embryos in short time.

Key words: Mice embryo, Vapor nitrogen jar, Cryopreservation

陈禹雷1，陈浩杰2，刘丽均1，赵丹凤1，徐平1. 气相液氮运输罐短期储存胚胎的可行性研究[J]. , 2008, 28(1): 18-22.

CHEN Yu-lei1,CHEN Hao-jie2,LIU Li-jun1,ZHAO Dan-feng1,XU Ping1. Saving Mice Embryos in Short Time by Using Vapour Nitrogen Jar[J]. , 2008, 28(1): 18-22.

[1]	冯玉芳1，陶凌云2，3，邵伟娟4，高诚2，3. 体外受精对小鼠早期生长发育的影响及性成熟期血清生化指标的分析[J]. , 2008, 28(2): 118-120.
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[3]	柴世庆1，2，刘丽均2，郁丽丽2，柴家前1，徐平2. 不同玻璃化冷冻方法对实验兔胚胎超低温保存的影响[J]. , 2007, 27(2): 90-93.
[4]	叶新红，严敬明，徐国江. 羊输卵管上皮细胞对小鼠受精卵体外发育的影响[J]. , 2001, 21(1): 17-21.
[5]	刘丽均, 徐平. 小鼠桑椹期、囊胚期胚胎移植方法的探讨[J]. , 2000, 20(2): 109-110.

气相液氮运输罐短期储存胚胎的可行性研究

Saving Mice Embryos in Short Time by Using Vapour Nitrogen Jar

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