关键词: 食管癌, 动物模型, 肿瘤转移, 细胞系, 细胞亚系

Abstract: Objective To report a new method for the establishing spontaneously metastatic animal model of human esophagus carcinoma and the metastatic-lymph node-deriving subline. Methods SCID mice were used in the experiments. Human esophagus carcinoma cell line Eca-109 were injected into the gastric mucosa layers in mice with a concentration of (1-2) X 10⁶ in 0.2 ml solution. All mice were sacrifed 3 months later or when the mice were found under the condition of general failure. Tumor metastases were investigated pathologically，part of the metastastic lymph node were inoculated subcu-taneously for the establishment of metastatic sub line. Results We established 9 gastric implantation animal models, one of which were found to have lung and lymph node metastases. Part of the metastatic lymph node was implanted sub cutaneous ly in mice, and after its survival in vivo for 4 passages, a new subline was established using primary tissue culture. Conclusion Gastric wall implantation is a feasible method for establishing spontaneous metastases animal model and metastatic sub line of human esophagus carcinoma.

Key words: Esophagus carcinoma, Animal model, Tumor metastasis, Cell line, Subline