大鼠膀胱癌模型建立的实验研究

›› 2007, Vol. 27 ›› Issue (1): 32-36.

大鼠膀胱癌模型建立的实验研究

1.苏州大学第三附属医院、常州市第一人民医院泌尿外科,常州 213003；2.南京医科大学第一附属医院泌尿外科,南京 210029

收稿日期:2006-06-28 出版日期:2007-01-31 发布日期:2007-01-31
基金资助:
江苏省“135工程”重点医学课题资助（RC2002051）

Experimental Study on Bladder Tumor Model in Wistar Rats

1. Department of Urology, the Third Affiliated Hospital, Suzhou University, Changzhou 213003, China;2. Department of Urology, the First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China

Received:2006-06-28 Online:2007-01-31 Published:2007-01-31

摘要/Abstract

摘要： 目的动态观察诱癌剂N-甲基-N-亚硝基脲（MNU）诱发Wistar大鼠膀胱肿瘤发生发展的过程，探讨建立膀胱癌动物模型的合适方法.方法 60只Wistar大鼠随机分成3组，用MNU（2 mg/2周）定期作膀胱内灌注，实验周期为14周，按计划处死大鼠，观察不同阶段动物膀胱癌的发生情况.结果 60只大鼠中58只完成了实验，2只中途死亡，诱癌组（MNU组）存活39只大鼠中，病理证实为膀胱癌者37只，占92.5%，2只为不典型增生，占5%.在病理确诊的39只大鼠中，有33只联合使用尿HE染色+AO染色诊断为膀胱肿瘤，阳性率89.2%.结论 MNU经膀胱灌注诱发膀胱肿瘤动物模型方法简单、药物用量少、诱瘤时间短、致癌率高，其诱发的膀胱肿瘤在组织学和病理学特征上和人的膀胱肿瘤十分相似，是较理想的动物模型.在动物实验中用尿HE染色+AO染色诊断膀胱肿瘤有一定应用价值.

关键词: 膀胱肿瘤, 膀胱内灌注, 诱癌剂, N-甲基-N-亚硝基脲(MNU)

Abstract: Objective To study the dynamic processes of bladder tumor in Wistar rat induced by intravesical irrigation of the carcinogen,MNU and discuss how to establish an appropriate animal model of bladder tumor. Methods Sixty Wistar rats were randomly divided into 3 groups. The model rats were intravesically affused with MNU as carcinogen to induce bladder tumor every two weeks. Rats were sacrificed in different periods. Results In total of 60 rats , 58 of them had finished the experiment while 2 rats died during the period.In cancer induced group(VINU group) there were 39 survived during the experiment in which 37(92.5%) of them were confirmed by pathology to be bladder cancer and other 2 rats(5%) were atypical dysplasia(hyperplasia).Thirty three rats were diagnosed to be positive bladder tumor in 37 rats combined with urinary HE and AO pigmentation while the positive ratio is 89.2%. Conclusion Bladder tumor induced by MNU is highly resemble with that of human beings in both histologic and pathologic character.lt is expected to have good foreground to diagnose bladder tumor in animal model combined with urinary HE and AO pigmentation.

Key words: Bladder tumor, Intravesical irrigation, Carcinogen, N-methel-nitrosourea(MNU)

丁鸿1，钱立新2. 大鼠膀胱癌模型建立的实验研究[J]. , 2007, 27(1): 32-36.

DING Hong1;QIAN Li-xin2. Experimental Study on Bladder Tumor Model in Wistar Rats[J]. , 2007, 27(1): 32-36.