rhBMP-2在诱导C3H10T1/2干细胞成骨中对MMP-13、Collagen-2 mRNA表达的影响

›› 2007, Vol. 27 ›› Issue (1): 29-31.

rhBMP-2在诱导C3H10T1/2干细胞成骨中对MMP-13、Collagen-2 mRNA表达的影响

苏州大学医学院生化与分子生物学教研室，苏州大学生化工程研究所，苏州 215123

收稿日期:2006-06-28 出版日期:2007-01-31 发布日期:2007-01-31
基金资助:
国防科工委国防基础科研项目（Kolo2061501）；江苏省高校自然科学研究基金课题资助项目（04KJB320129）；苏州大学医学发展基金（2007）

The Effect of rhBMP-2 on the Expression of MMP13 and Collagen-2 in mRNA Level during Osteogenic Differentiation

Department of Biochemistry and Molecular biology, Suzhou University, Suzhou 215123, China

Received:2006-06-28 Online:2007-01-31 Published:2007-01-31

摘要/Abstract

摘要： 目的研究基质金属蛋白酶13（MMP13）、Ⅱ型胶原（co1lagen2）在C3H10T1/2细胞中的相互关系，探讨rhBMP-2在诱导C3H10T1/2成骨的同时对MMP13以及Collagen2 mRNA表达水平的影响.方法取C3H10T1/2细胞加入rhBMP-2诱导成骨细胞的产生，Von Kossa染色观察细胞形态，同时在不同时间段抽取总RNA，利用Real-time PCR检测MMP13、Collagen2的表达情况.结果未加rhBMP-2的细胞中MMP13、Collagen2前10d表达呈负相关；而rhBMP-2处理后的细胞中MMP-13表达水平受抑制，且MMP13和collagen2前14d表达呈明显负相关.结论 MMP-13和Collagen-2 mRNA表达水平具明显相关性，rhBMP-2在诱导成骨的同时，具有抑制MMP-13表达的作用.

关键词: MMP-13, Collagen 2, rhBMP-2, Real-time PCR

Abstract: Objective To investigate the interaction of metalloproteinase-13 (MMP-13) and type II collagen (Col-2) in C3H10T1/2 cells, and explore the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on the expression of MMP13 and Col-2 in mRNA level during osteogenic differentiation induced by rhBMP-2. Methods rhBMP was utilized to induce osteoblastic differentiation of C3H10T1/2 cells. Total RNA was isolated at various time points to detect the expression of MMP13 and Col-2 by the method of quantitative Real-time PCR. Results There was a negative correlation between MMP-13 and Col-2 expression in C3H1 OTI/2 cells without rhBMP-2 treatment in the first 10 days. In the rhBMP-2 treated cells, however, expression of MMIP-13 was inhibited and there was a negative correlation between MMP13 and Col-2 expression in the first 14 days. Conclusions There is a obvious correlation between the expression of MMP13 and Col-2. rhBMP-2 inhibits the expression of MMP13 while it induces osteogenic differentiation of C3H10T1/2 cells.

Key words: MMP-13, Collagen-2, rhBMP-2, Real-time PCR

徐岚，范雁，周迎会，吴士良. rhBMP-2在诱导C3H10T1/2干细胞成骨中对MMP-13、Collagen-2 mRNA表达的影响[J]. , 2007, 27(1): 29-31.

XU Lan;FAN Yan;ZHOU Ying-hui;WU Shi-liang. The Effect of rhBMP-2 on the Expression of MMP13 and Collagen-2 in mRNA Level during Osteogenic Differentiation[J]. , 2007, 27(1): 29-31.