大鼠精子冷冻保存和卵胞浆内精子注射研究进展

实验动物与比较医学 ›› 2006, Vol. 26 ›› Issue (4): 253-256.

• 论文 •

大鼠精子冷冻保存和卵胞浆内精子注射研究进展

1.扬州大学比较医学中心，扬州 225009；2.中国科学院上海实验动物中心，上海 201615；3.南京农业大学动物医学院，南京 210095；4.沈阳农业大学畜牧兽医学院，沈阳 110161

基金资助:
上海市科委科研基金项目（课题编号 054919007）

Advances on Sperm Cryopreservation and Intracytoplasmic Sperm Injection in Rat

1.Comparative Medicine Center Of Yangzhou University, Yangzhou 225009,China；2. Shanghai Laboratory Animal Center, Chinese Academy Of Sciences, Shanghai 201615,China；3 . Veterinary College of Nanjing Agriculture University, Nanjing 210095,China；4.College of Animal Science and Veterinary,Shenyang Agriculture University, Shenyang 110161,China

摘要/Abstract

摘要： 许多哺乳类动物精子冷冻保存技术均已成功建立。然而，成功建立一套啮齿类动物精子冷冻保存程序还具有一定的挑战性。尤其是大鼠，因为其较长的精子尾巴，使得它的精子冷冻保存相当困难，并且大鼠的卵胞浆内精子注射（ICSI）技术也没有小鼠那样成熟。国内尚未见关于大鼠精子冷冻保存和ICSI的报道，国际上关于这方面的报道也较少。本文就这两方面的研究进展做一简要综述。

Abstract: Sperm cryopreservation is a well-established technique for most mammals. Whereas, a successful cryopreservation protocol of rodent sperm remains a scientific challenge. Especially for rat, its long tail of sperm makes its sperm cryopreservation very hard. Accordingly，ICSI of rat is not done better than mouse. There are not any reports on rat sperm cryopreservation and Intracytoplasmic sperm injection（ICSI） in China, and the researches were not done widely abroard .The artical made a simple review on the advances of sperm cryopreservation and Intracytoplasmic sperm injection（ICSI） in rat.

. Advances on Sperm Cryopreservation and Intracytoplasmic Sperm Injection in Rat[J]. Laboratory Animal and Comparative Medicine, 2006, 26(4): 253-256.

大鼠精子冷冻保存和卵胞浆内精子注射研究进展

Advances on Sperm Cryopreservation and Intracytoplasmic Sperm Injection in Rat

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