›› 2003, Vol. 23 ›› Issue (1): 26-35.

• 论文 • 上一篇    下一篇

橡胶籽油对大鼠胚胎发育的影响

  

  1. 上海市实验动物质量监督检测站,上海 200032
  • 收稿日期:2002-07-25 出版日期:2003-01-25 发布日期:2013-03-19

Effects of Rubber Plant Seed Oil on Rat Embryo Development

Department of laboratory animal science, Fudan University, Shanghai 200032,China   

  1. Shanghai Quality Monitoring Center for Laboratory Animals,Shanghai 200032 ,China
  • Received:2002-07-25 Online:2003-01-25 Published:2013-03-19

摘要: 为研究橡胶籽油对SD大鼠胚胎发育的影响,将60只妊娠SD大鼠随机分成阴性对照组、阳性对照组和高中低剂量处理组。孕鼠于妊娠7~16d分别灌服植物油、敌枯双和高中低剂量橡胶籽油。妊娠0、7、12、16、20 d称重。妊娠20d处死剖腹取子宫,检查并记录着床数、吸收胎、死胎和活胎数;活胎鼠观测胎盘、胎仔体重、体长、尾长和外观。结果表明:阳性对照组孕鼠体重增长明显低于阴性对照组、剂量处理组(P<0.05),而剂量处理组和阴性对照组相比无差异(P<0.05);活胎仔数、吸收胎数、死胎数、平均胎仔体重、体长及尾长,阳性对照组与阴性对照组、剂量处理组相比有显著差异(P<0.05),而剂量处理组与阴性对照组无差异(P<0.05)。结果提示,橡胶籽油对SD大鼠胚胎发育无明显致畸和胚胎毒性作用。

关键词: 随机扩增多态性DNA(RAPD)分析, 近交系大鼠, 遗传检测

Abstract: Nine random primers were used to amplify genomic DNA with randomly amplified polymorphic DNA (RAPD) methods in 3 inbred strains of rats. The result showed that the amplified band patterns of every individuals were same in one strain,the 3 strains of rats were homogeneous at these RAPD markers, however, the amplified band patterns showed different among three strains. The similarities between Wistar arid WKY, Wistar and F344,WKY and F344 were 87. 9%、98.5% and 89.2% respectively. The result indicated that the RAPD technique can be used as a method to monitor genetic contamination in inbred rats and to distinguish different strains of rats.

Key words: Rubber plant seed oil , Rats, Teratogenic effects, Embryo development