›› 2003, Vol. 23 ›› Issue (1): 23-25.
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上海市科委资助项目(994919038)
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摘要： 选用9条随机引物，对Wistar、WKY、F344三个品系的近交系大鼠进行了随机扩增多态性DNA（RAPD）分析，结果显示同一品系大鼠不同个体之间的RAPD图谱都相同，表明所测的三个品系大鼠内部遗传背景均一，在不同品系大鼠之间的RAPD结果有差异，Wistar与WKY之间、Wistar与F344之间、WKY与F344之间的共享度分别为87.9%、98.5%和89.2%。RAPD方法可以作为一种检测近交系大鼠种群内的遗传变异及区分不同品系近交系鼠的遗传检测方法。
关键词: 随机扩增多态性DNA(RAPD)分析, 近交系大鼠, 遗传检测
Abstract: Nine random primers were used to amplify genomic DNA with randomly amplified polymorphic DNA （RAPD） methods in 3 inbred strains of rats. The result showed that the amplified band patterns of every individuals were same in one strain，the 3 strains of rats were homogeneous at these RAPD markers, however, the amplified band patterns showed different among three strains. The similarities between Wistar arid WKY, Wistar and F344,WKY and F344 were 87. 9%、98.5% and 89.2% respectively. The result indicated that the RAPD technique can be used as a method to monitor genetic contamination in inbred rats and to distinguish different strains of rats.
Key words: Randomly amplified polymorphic DNA （RAPD）, Inbred strain rat, Genetic monitoring
谢建云，邵伟娟，王胜昌，高诚. 三个不同品系近交系大鼠的RAPD分析及比较[J]. , 2003, 23(1): 23-25.
XIE Jian-yun,SHAO Wei-juan，Wang Sheng-chang,GAO Cheng. RAPD Analysis and Comparative Study on Three Inbred Strains of Rats[J]. , 2003, 23(1): 23-25.
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https://www.slarc.org.cn/dwyx/CN/Y2003/V23/I1/23
