Table of Content

31 January 2008, Volume 28 Issue 1

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Effects of Rat Myocardial Infarction Models by Different Occlusion Time Course of LAD on Infarction Size and Heart Function

YANG Jian-ye1,ZHANG Ying-chun2,TANG Jun-ming1,3,PAN Guo-dong1,WANG Jian-ning1

2008, 28(1):  4-9. 

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Objective To study the relation of cardiac function and myocardial infarction size, which is induced by different occlusion time course of left anterior descending (LAD) in rats. Methods Myocardial infarction was induced by LAD occlusion in male Wistar rats. There is 60 male Wistar rats were randomly divided into six groups: acute LAD occlusion (15，30，45 or 60 min) with reperfusion or permanent LAD occlusion group. 4 weeks after different LAD ligation treatment, pathological morphology, IS and mortality was be detected and analyzed. And 1 month Afler LAD occlusion, we used multipurpose polygraph to measure heart rate(HR), left ventricular systolic pressure (LVSP), left ventricular end-diastolic pres sure (LVEDP), 士 dp/dt_max，artery systolic pressure(ASP), artery diastolic pressure(ADP). Results The myocardial necrosis or infarction was formed by LAD occlusion for at least 30 minutes. Almost the same infarct area had formed in rats among occlusion (45 or 60 min) with reperfusion groups, or permanent occlusion group. However, when arterial blood flow was restored (occlusion with reperfusion), the scattered bleeding and cell damage in rat myocardial infarction area occurred. After permanent LAD occlusion or occlusion reperfusion, nearly 25% rats died of ventricular fibrillation within 4 hours, and most of them died within the first 30 minutes. The heart sections from rats of one month after LAD occlusion showed the typical acute myocardial infarction pathological morphology. Compared to the LAD occlusion (15 or 30 min) with reperfusion group, ASP, LVSP and 土 dp/dt_max of the LAD occlusion(45 or 60 min) with reperfusion or permanent LAD occlusion group were decreased, LVEDP increased. Conclusion The rat acute myocardial infarction model is the most stable by LAD occlusion (at least 45 minutes) with reperfusion and the change of cardiac function is related with the size of myocardial infarction areas after LAD occlusion with reperfusion.

The Valuation of Postresuscitation Myocardial Function after Ventricular Fibrillation by Echocardiography

LI Song-hua1,YANG Jun-hua1,WANG Yu-bin2,ZHOU Zheng-yu2,ZHOU Hui-ying2,HUANG Men2,YIN Yin2,LIU Hui-ting2,GU Jing2,XUE Zhi-mou2

2008, 28(1):  10-13. 

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Objective To investigate the functional changes in the myocardium afler successful resuscitation from ventricular fibrillation(VF). Methods Ventricular fibrillation was induced in Taihu-pigs with an alternating current(AC) delivered to the right ventricular endocardium. Afler either 2 or 6 minutes of untreated ventricular fibrillation,the animals were attempted to defibrillate. Then transthoracic echocardiography was applied to evaluate myocardial function after successful resuscitation. Results Myocardial dysfunction of all animals after successful resuscitation were observed,which had remarkable improvement when they survived more than 72 hours. Myocardial function of the animals afler 6 min of untreated ventricular fibrillation had more serious impairment than that after 2 minutes(P＜0.05). Conclusion As the duration of untreated ventricular fibrillation was prolonged,the severity of postresuscitation myocardial dysfunction would be increased. The myocardial dysfunction with left ventricle dilation may be reversible probably.

Saving Mice Embryos in Short Time by Using Vapour Nitrogen Jar

ZHANG Yu-ying,SHEN Xin-e,ZOU Rong,LI Jin-hua,WANG Guo-qing

2008, 28(1):  14-17. 

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Object Studing the feasibility of saving mice embryos in vapour nitrogen transport jar in short time. Method we used in vitro fertilization to get the 2-cell embryos of C57BL/6J, B6D2F1, DBA/2, B10A，these embryos were vitrificated in liquid nitrogen，then transferred to vapour nitrogen jar. About 14 days later，these embryos were transferred to liquid nitrogen again for some time. Last, these embryos were post-thawed and transferred to psudopregnant mice. Result The rate of the post-thawmg of the embryos from C57BL/6J，B6D2F1, DBA/2, B10A were 69.70%, 77.27%, 77.59%, 72.64%, and the control groups (embryos vitrificated in liquid nitrogen only) were 97.00%、84.38%、 75.00%、71.43% respectively, there was no difference in the two groups (P＜0.05). When transferred to psudopregnant, there were no difference in C57BL/6J, B6D2F1, DBA/2 in tne rate of birth. But for B10A，the difference was significant(P＜0.01). Conclution The vapour nitrogen transport jar is suitable to cryopreserve and transport mice embryos in short time.

Saving Mice Embryos in Short Time by Using Vapour Nitrogen Jar

CHEN Yu-lei1,CHEN Hao-jie2,LIU Li-jun1,ZHAO Dan-feng1,XU Ping1

2008, 28(1):  18-22. 

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Object Studing the feasibility of saving mice embryos in vapour nitrogen transport jar in short time. Method we used in vitro fertilization to get the 2-cell embryos of C57BL/6J, B6D2F1, DBA/2, B10A，these embryos were vitrificated in liquid nitrogen，then transferred to vapour nitrogen jar. About 14 days later，these embryos were transferred to liquid nitrogen again for some time. Last, these embryos were post-thawed and transferred to psudopregnant mice. Result The rate of the post-thawmg of the embryos from C57BL/6J，B6D2F1, DBA/2, B10A were 69.70%, 77.27%, 77.59%, 72.64%, and the control groups (embryos vitrificated in liquid nitrogen only) were 97.00%、84.38%、 75.00%、71.43% respectively, there was no difference in the two groups (P＜0.05). When transferred to psudopregnant, there were no difference in C57BL/6J, B6D2F1, DBA/2 in tne rate of birth. But for B10A，the difference was significant(P＜0.01). Conclution The vapour nitrogen transport jar is suitable to cryopreserve and transport mice embryos in short time.

The Comparison of Different Methods on Flow Cytometric Analysis of Activated Platelet

WANG Zhi-jun1,ZHAN Yu-liang2,CHENG Wen-li2,YU Chang-an2,KE Yuan-nan2

2008, 28(1):  23-26. 

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Objective To investigate the effect of different methods on flow cytometric analysis of activated platelet. Methods Eight Wistar rats were randomly chosed and blood preparation drawed from abdominal aorta. Each specimen was dealed with conventional whole blood method, internal fixative whole blood method, external fixative whole blood method，platelet rich plasma (PRP) first fixative method, and PRP lastly fixative method respectively. Flow cytometric analysis was applied to detect the expression of CD62 on the surface of platelet.The database was established by Excel software and analyzed by SPSS 11.5 software. Results The expression of CD62 on the surface of platelet of different method were not coherent. The increase progressively sequence was internal fixative whole blood method, external fixative whole blood method, conventional whole blood method, PRP first fixative method and PRP lastly fixative method. There was obviously distinction between every methods. Conclusion Internal fixative whole blood method fixed platelet promptly and avoided platelet activation out-of-body, so it could reflect actual activated degree of platelet.

The Influence of Iradiate Injury on VEGF Expression in Different Organ of Mice

SUN Bin1,YANG Zhan-san2,ZHOU Zhen-yu3,JIN Mei-fang1

2008, 28(1):  27-29. 

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Objective To study the expression and the meaning of the vascular endothelial growth factor(VEGF) mRNA in the lung, thymus, intestines and spleen after suffering from radiative injury.Methods The mice were radiated with different doses of X ray (0,4,8 Gy) and the expression of VEGF mRNA in the lung, thymus, intestines and the spleen after 24 hours’ radiation were detected by PT-PCR. Results The expressions of VEGF mRNA in intestines and lung were found to be no significantly difference (P＞0.05) with those in normol control groups after radiated by 4 and 8 Gy ray. The expression of VEGF mRNA in the spleen and thymus gland were higher than those in normol control groups. The expressions of VEGF mRNA in the groups which radiated by 4 Gy were significantly higher than others (P＜0.01). Conclusions The expression level of VEGF mRNA become higher after different radiation dose in the spleen and thymus gland，but have no significant difference in the intestine and lung. Thus VEGF may has stronger radiative protection on immune system than in other organizations, which provides a theoratical basis for treatment of radiative injury.

Murine Models of Candida Infection and Host Genetic Factors

ZHANG Xiao-huan1,HU Yan1,Camile S Farah2,Robert B Ashman2

2008, 28(1):  57-61. 

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Infections caused by Candida albicans can occur on the skin, on mucous surfaces, or in disseminated form throughout the body. The models on these infections have been developed in vivo and in vitro. Patterns of infection differ in inbred mice, and the variability has been shown to be genetically regulated for both experimental systems. Fortunately, the availability of gene-knockout mice has also contributed significantly to an understanding of the cellular and molecular basis of the host response. In this article, the links between the patterns of infection and the genetic factors that affected the infection was reviewed for a better elucidation on this issue.

Modeling Liver Fibrosis in Rats

KUANG Sheng-li,HU Bing

2008, 28(1):  62-66. 

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Liver fibrosis is the excessive accumulation of extracellular matrix proteins including collagen that occurs in most types of chronic liver diseases. Advanced liver fibrosis results in cirrhosis and liver failure. Animal models of liver fibrosis provide a means to study the cell and molecular mediators of fibrosis in a serial manner during both progression and recovery. This review summarizes several approaches to induce rats liver fibrosis. Detailed Method, pathogenesis, pathology changes of liver, advantages and disadvantages of each method and different application in research were discussed.