30 April 2007, Volume 27 Issue 4

    

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  Endogenous Circadian Expression of the Arylalkylamine N-acetyltransferase Gene in the Pineal Gland of Rat

  WANG Guo-qing1,2,FU Chun-ling2,LI Jian-xiang2,DU Yu-zhen2,TONG Jian2

  Laboratory Animal and Comparative Medicine. 2007, 27(4): 215-218.

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  Objective To investigate the endogenous circadian expression of the Arylalkylamine N-acetyltransferase（NAT）gene in the rat pineal gland.Methods 36 SD rats under constant darkness（DD）for eight weeks were sampled for the pineal gland once every 4 hours in a circadian day.The total RNA was extracted from the samples and the semi quantitative reverse transcription polymerase chain reaction（RT-PCR）was used to determine the temporal changes in mRNA level of the NAT gene during different circadian times（CT）.The data parameters were obtained and analysed by both the cosine function software and the amplitude F test to reveal the circadian rhythm.Results The NAT mRNA level in the pineal gland showed a significantly circadian oscillation（〈i>P
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  Evaluation and Establishment of Endometriosis Model by using Transgenic Mice Expressing GFP

  CHENG Ming-jun1,YU Er-kai1,GUO Fang1,WANG Yi-sen2,XU Cong-jian1

  Laboratory Animal and Comparative Medicine. 2007, 27(4): 219-221.

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  Objective To develop the endometriosis model in the mice, and to investigate the methods of analyzing the ectopic endometrium using the fluorescent imaging. Methods Endometrial fragment obtained from the trans gene tic mice treated by Green Fluorescent Protein (GFP) and injected into the peritoneal cavity of C57BL/6 mice. These recipient mice were raised with water supplemented by E₂ for three weeks. Then the mice were euthanized,and we observed the intensity of the GFP of the peritoneal cavity under fluorescence microscopy. Results The green fluorescence does exist in the peritoneal cavity of the mice and we have proved that the ectopic endometrium expressed the GFP by the immunohistochemistry. Conclusion The model developed by using the transgeneic mice expressing GFP would be available for exact evaluation of endometriotic lesions, which is prospective.
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  Establishing Modified Model of Chronic Aristolochic Acid Nephropathy In Rats

  WANG Yun-man1,HE Li-qun2,CHEN Ling3

  Laboratory Animal and Comparative Medicine. 2007, 27(4): 222-225.

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  Objective To establish chronic aristolochic acid (AA) nephropathy in rats. Methods Male SD rats were randomly divided into control and model group. The model group rats were given daily with AA 5 mg/kg intragastically，the control group rats were given equal amount of normal saline. The 24h urine and blood samples were collected for detection of indexes of renal function, and at the end of the 8th week, take their nephridial tissue to test the change of pathology. Result Along with feeding times，the level of the urine β₂-MG and NAG enzyme exertion increased markedly and the damage of tubular inters titium after giving AA in tset groups. Conclusion The model of chronic A AN in rat had been finished basically.
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  Influence of Insulin on Mouse Embryo and IGF-1 Express in their Tissues

  GAO Jun1,3,SHAO Wei-juan2,TAO Lin-yun2,SUN Feng-ping1,HU Jian-hua2,XUE Zhi-mou3

  Laboratory Animal and Comparative Medicine. 2007, 27(4): 226-229.

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  Objective To evaluate the influence of the insulin on the development of mouse preimplantation embryo and try to find the IGF-1 express situation in mouse tissues. Method Two-cell mouse embryos, collected from oviduct flushing，were divided into two groups, one group of embryo were cultured for 36 hours in the KSOM media supplemented with insulin at concentration of 0.25 ug/ml as insulin group (Ins group). Another group of embryo was cultured in the same KSOM media but without insulin as control group. After the embryo cultured to blastocysts in vitro, two groups blastocysts were transplanted to the uterine of induced pseudopregnant ICR mouse .The birth weight of the FI fetal mouse were measured. The IGF-1 mRNA express of two group fetal mouse in their three kinds of tissues (liver、muscle and brain) were detected by half-quantities RT-PCR method. Result The rate of Ins-group development to blastocyst after the cultured 36h is higher than that of control group about 9.7%. The average body weight of FI fetal mouse in Ins-group is higher than that of the control group about 17.8% (P＜0.01). Half-quantities RT-PCR results also showed that IGF-1 express level in the muscle of FI Ins-group is higher than that of Control group. But no IGF-1 express difference was found in the liver and brain Conclusions The suitable concentration of insulin in the media supplement in vitro can accelerate the embryo development and increase the IGF-1 mRNA express in muscle of the fetal mouse.
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  Comparative Medicine Study on Male Fishar344 Rats Spontaneous Leydig cell tumor

  WANG Yi-sen1,ZHU Ping-mei2,ZHONG Gao-ren3,XU ping4,SHI En4,QIAO Wei-wei2,ZHOU Guang-xing2

  Laboratory Animal and Comparative Medicine. 2007, 27(4): 230-235.

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  Objective To explore the relationship between Leydig cell tumor and levels of sex hormones and the expression levels of AR，LH and ER in Leydig cells in male Fisher344 rats.Methods Male Fisher344，Sprague D awl eg，Wistar rats were respectively divided into 4-month-old group（group A₁，B₁，C₁），7-mo nth-old group（group A₂，B₂，C₂），10-month-old group（group A₃，B₃，C₃），13-month-old group（group A₄，B₄，C₄）.Each group comprised of ten rats.In addition，another group was five 26-month-old male Fisher344 rats（group L）.Pathologic changes in testes were observed with microscope.Testes of group L were observed with transmission electron microscope.Immuno-histochemistry analysis of androgen receptor，luteinizing hormone，estrogen receptor were taken in tests by S ABC method，in every group except group L.The levels of testosterone（T），luteinizing hormone（LH），estradiol（E2），prolactin（PRL），follicle-stimulating hormone（FSH）and progesterone（P）in sera of each group were measured with radioimmunological analysis method.Results Hyperplasia of Leydig cell occurred in group A₃，and more hyperplasia in group A₄，and focal hyperplasia had formatted in some cases.All testes of group L had generated Leydig cell tumor.The expression level of AR in Leydig cells in group A₄ was higher than that of group C₃，B₄，C₄（P＜0.05）.The expression levels of LH in Leydig cell in group A₃ and A₄ were more than that of group B₃，B₄ and C₄（P＜0.05）.The expression level of ER in Leydig cell in 1 group A₄ was less than that of group Bi，B₄ and C₄（P＜0.05）.The level of sera T of group A₃ or A₄ was higher than that of group B₃，B₄，C₃ or C₄（P＜0.01）.Group L has the lowest level of sera T and it was lower than that of the other groups（P＜0.05 or P＜0.01）except group C₄（p≥0.05）.There were no significant differences between level of sera LH group L and that of group A₄，B₄，C₄（≥0.05）.The level of sera E2 of group L was the highest，which was significantly higher than that of the other groups（P＜0.05）except group Bi and B₂（p≥0.05）.The level of sera PRL of group L was significantly higher than that of group A₄（P＜0.05），and one of both groups was respectively higher than other groups（P＜0.01）.There is no significant difference of the level of sera FSH of the same age groups（P≥0.05），and the levels of sera FSH cf group L，A₄，B₄ and C₄ were almost the same.The level of sera P of group L was significantly higher than that of the other groups（P＜0.01）.Conclusions Most of the Leydig cell tumors in male Fisher344 rats were benign tumor.Leydig cell tumor in male Fisher344 rats may be developed from hyperplasia and focal hyperplasia of Leydig cell.There was a significant correlation between the relatively more expression level of LH and AR in Leydig cell and hyperplasia and focal hyperplasia of Leydig cell in male Fisher344 rats.The development of spontaneous Leydig cell tumors in old male Fisher344 rats may be related to their extremely low level of sera T and the relatively low level sera E2 and P but uncorrelated with the level of sera FSH and LH.The relatively high level of sera PRL may be related to Leydig cell hyperplasia and tumor generation.
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  Research of Effective Fractions for Gout in Modified “Si miao Powder”

  SHI Le1,XU Li2,TAN Qiu Wei2,YIN Lian3

  Laboratory Animal and Comparative Medicine. 2007, 27(4): 236-238.

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  Objective To observe the function of anti-inflammation，abirritation and lowering uric acid of effective fractions in Modified “Si miao Powder”. Methods The pain reaction of mouse by chemical stimulation，the ear swelling of mouse induced by xylene and hyperlithuria model were adopted to observe the anti-inflammation，abirritation and lowering uric acid effect of Modified “Si miao Powder” and its effective fractions. Result Modified “Si miao Powder” can fall the uric acid in 3h to the hyperuricemia mouse (P＜0.05) and has obvious paregoric effect to the writhing response mouse stimulated by acetic acid (P＜0.01). The large dose of A fraction can obviously reduce the high uric acid level (P＜0.01)，It displays the obvious anti-inflammation and the analgesia function to ear swelling mouse by xylene and writhing response mouse by acetic acid (P＜0.01).In the A fraction the middle dosage group serum can reduce the mouse high uric acid level in 1 h，3 h (P＜0.05，P＜0.01). It has obvious inhibitory action to ear swelling mouse and writhing response mouse (P＜0.01).The low dosage group of the can suppress the serum uric acid (P＜0.05) and has obvious inhibitory action to ear swelling mouse (P＜0.01)， but has not displayed the analgesia function. Conclusion Effective fractions of the Modified “Si miao Powder” is the material basis, its groups showed some, dose-effect relationship i in ruducing uric acid analgesia, fall the uric acid and the anti-inflammation aspect.
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  Progress in Studies of Memory Impairment of Diabetes in Clinic and Animal Models

  QIAN Bo-chu1,2,SHI Hong1,ZHENG Xiao-liang1

  Laboratory Animal and Comparative Medicine. 2007, 27(4): 272-275.

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  Diabetes could conceivably lead to cognitive impairment through chronic hyperglycemia. Diabetes-induced learning and memory impairment is the most common serious complication, and its ponderance is emerging as a common medical problem. Along with the research and production of anti-diabetes drugs, the animal models are well developed. In this paper, we reviewed the progress in studies of preparation in animal models of strep to zotocin-induced diabetic rats by intraperitoneal injection or Intracerebroventricular injection，and in studies of diabetes-induced cognize disorder in clinic.
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  Structural Character and Detective Techniques of Murine Norovirus

  ZHAI Xin-yan,LU Sheng-ming

  Laboratory Animal and Comparative Medicine. 2007, 27(4): 276-279.

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  Murine Norovirus l(MNV-l) is a newly recognized pathogen of mice that causes lethal infection in mice deficient in components of the innate immune response. Moreover, the murine virus 1 is the only norovirus that can replicate in cell culture and in a small animal. Murine norovirus 1 is detected by virus isolation, immunohistochemistry method and RT-PCR technique.
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  The Progress of Research on Animals Superovulation Technology

  WANG Xin-lei1,SHAO Yi-xiang1,WANG Peng2

  Laboratory Animal and Comparative Medicine. 2007, 27(4): 280-284.

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  On account of the significancant role of the superovulation technique to prologue production and preservation for endangered species and biotechnology research as well as its special contribution to human assisted reproductive technique,this article summarizes the usages and characteristics of hormones used for superovulation technology in domestic and abroad,It also introduces the application of superovulation technology on different genus mammals and the recent development of this technology.