Abstract: Objective To investigate the effect of different methods on flow cytometric analysis of activated platelet. Methods Eight Wistar rats were randomly chosed and blood preparation drawed from abdominal aorta. Each specimen was dealed with conventional whole blood method, internal fixative whole blood method, external fixative whole blood method，platelet rich plasma (PRP) first fixative method, and PRP lastly fixative method respectively. Flow cytometric analysis was applied to detect the expression of CD62 on the surface of platelet.The database was established by Excel software and analyzed by SPSS 11.5 software. Results The expression of CD62 on the surface of platelet of different method were not coherent. The increase progressively sequence was internal fixative whole blood method, external fixative whole blood method, conventional whole blood method, PRP first fixative method and PRP lastly fixative method. There was obviously distinction between every methods. Conclusion Internal fixative whole blood method fixed platelet promptly and avoided platelet activation out-of-body, so it could reflect actual activated degree of platelet.

Key words: Activated platelet, Flow cytometric analysis, Detect methods