Abstract: Object Studing the feasibility of saving mice embryos in vapour nitrogen transport jar in short time. Method we used in vitro fertilization to get the 2-cell embryos of C57BL/6J, B6D2F1, DBA/2, B10A，these embryos were vitrificated in liquid nitrogen，then transferred to vapour nitrogen jar. About 14 days later，these embryos were transferred to liquid nitrogen again for some time. Last, these embryos were post-thawed and transferred to psudopregnant mice. Result The rate of the post-thawmg of the embryos from C57BL/6J，B6D2F1, DBA/2, B10A were 69.70%, 77.27%, 77.59%, 72.64%, and the control groups (embryos vitrificated in liquid nitrogen only) were 97.00%、84.38%、 75.00%、71.43% respectively, there was no difference in the two groups (P＜0.05). When transferred to psudopregnant, there were no difference in C57BL/6J, B6D2F1, DBA/2 in tne rate of birth. But for B10A，the difference was significant(P＜0.01). Conclution The vapour nitrogen transport jar is suitable to cryopreserve and transport mice embryos in short time.

Key words: Mice embryo, Vapor nitrogen jar, Cryopreservation