Effects of Irx5a Gene Overexpression on Early Hematopoietic in Zebrafish Embryos

doi:10.3969/j.issn.1674-5817.2017.04.011

Abstract

Abstract: Objective To investigate the expression and influence of early hematopoietic related genes in zebrafish embryos. Method The single-cell-stage zebrafish embryos were micro-injected with iroquois homebox 5a-enhanced green fluorescent protein (Irx5a-EGFP) mRNA as experimental group, EGFP mRNA as experimental control group, wild type zebrafish as blank control group. Comparised the hematopoietic transcript factor genes with three groups by quantificational reverse transcription-PCR (qPCR). Such as primitive hematopoiesis scl and lmo2, definitive hematopoiesis c-myb and runx1, erythroid hematopoiesis gata-1, myeloid hematopoiesis pu.1. The zebrafish embryos’ time point are 9 h、12 h、18 h、24 h、30 h post fertilization (pf). Results From the real-time fluorescence quantitative PCR can be seen in the original hematopoietic transcription factor lmo2 and scl, the experimental group from 9 hpf to 24 hpf significantly lower than the other two groups. In the targeted hematopoietic transcription factor, c-myb experimental group from 12 hpf to 24 hpf significantly lower than the other two groups, runx1 experimental group from 9 hpf to 30 hpf significantly lower than the other two groups. Myeloid hematopoietic transcription factor pu.1 and erythroid hematopoietic transcription factor gata-1, the experimental group from 9 hpf to 30 hpf were significantly lower than the other two groups. The differences were statistically significant. Conclusion Irx5a gene plays an inhibitory role in the transcriptional regulators of primitive hematopoiesis, adult hematopoiesis, erythroid hematopoiesis and myeloid hematopoiesis in the zebrafish.

Key words: Zebrafish, Iroquois homebox 5a(Irx5a), Hematopoietic transcription factor

CLC Number:

Q95-33

JIN Lu, LI Yan, LI Zhi-cao, WU Xi-jun, ZHOU Yan-hua, HE Zhi-xu, SHU Li-ping. Effects of Irx5a Gene Overexpression on Early Hematopoietic in Zebrafish Embryos[J]. Laboratory Animal and Comparative Medicine, 2017, 37(4): 309-314.

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