Abstract: Objective To detect the immunogenicity of NP gene of RV. Method NP gene of RV was amplified by RT-PCR and then cloned into plasmid pVAXl to construct recombinant plasmid pVAXl-NP.The Vero cells were transfected with plasmid pVAXl-NP. Expression of the NP protein was detected by SDS-PAGE and Westem-blot assay. The mice were immuned with plasmid pVAXl-NP. Result The plasmid PVAXl-NP was obtained and the NP gene was expressed in Vero cells .The expression products had immunogenic ity and the antibody level of immuned mice was higher. Conclusion The NP protein of RV had immunogenic ity.

Key words: RV, NP gene, Eukaryotic expression