Abstract: Objective Using RA and DMSO to induce porcine embryonic stem cells, they were certificated that could be differentiated into the various kinds cell. Methods Porcine embryonic stem cells were induced to embryonic bodies in culture of free feed or culture of suspension. Embryonic bodies were cultured in the medium with RA or DMSO respectively, to induce embryonic stem cell to differentiate. Results The embryonic bodies would differentiate into neuron-like cells, trophoblast-1 ike cells, morula-like colonies and ES-like colonies when they cultured in the medium with RA and DMSO. Conclusion Porcine EG cells could be differentiated into the various kinds cell. It was testified that porcine EG cells were pluripotency.

Key words: Pig, Embryonic stem cell, Differentiation, Retinoic acid, Dimethylsulphoxide