Abstract: To examine whether rat osteoportic model was established quickly by high protein and low calcium diets, Forty female Wistar rats （3 weeks old）were randomly divided into 5 Groups and fed with diets formulated according to AO AC for twenty weeks. The contents of protein and calcium in 5 Groups were 18%,1%；8%, 0.025%；8%；1%； 27%,0.025%；and27%，1% respectively. The results showed that the content of bone calcium in Group 2 and Group 4 was obviously decreased compared with that of Group 1 （P＜0. 001）, the BMD and hending stress resistance of femur was also decreased （P＜0.01）, and the trabecular area of lumbar and tibia was increased. In compared with Group 1, The body weight gain of Group 2 was obviously decreased （P ＜0. 001） and estradion in serum was decreased （P＜0.05）. The results suggested that the Rat osteoportic model established by high protein and low calcium diets for twenty weeks was successful.

Key words: Osteoporosis, High protein, Low calcium, Rats, Model, Dietary