Abstract: To establish a model of acute hemorrhagic-necrotizing pancreatitis （AHNP） in rats for evaluating therapy, the rats were chalanged by homogeneous injection of 1 ml of 3 percent sodium taurocholate beneath the pancreatic capsule. The results showed that pancreatic hemorrhage and necrosis were observed within half an hour of the operation.Serum amylase and pancreatic tissue TNFα levels were peaked 24 hours later and maintained for 24 hours. Histological injury and progressively increased TNFa levels of liver and lungs were observed subsequently. Two-day mortality of this model was zero. The mortality was gradually increased and reached to 90% after one week. The results suggested that the AHNP rat models established were accompanied by multiply organ injury. The severity of AHNP is progressive with finite one-week mortality rate. The model resembles the natural course of human severe acute pancreatitis （SAP） and may be suitable for evaluating therapeutic study on SAP.

Key words: Pancreatitis, Animal models, Rat