Abstract: Double fluorescence immunostain of tyrosine hydroxylase （TH） and glutamate （Glu） was used in midbrain sections of rat.With modified procedure of brain perfusion for reducing background of Glu staining,we got satisfied results,and found that 91%±4% of DA neurons were Glu positive.Cytohistological studies demonstrated that most ventral midbrain DA neurons were glutamatergic.

Key words: Double fluorescence immunostaining, Rat, Tyrosine hydroxylase, Glutamate