Laboratory Animal and Comparative Medicine

In Utero Ethanol Exposure Affects the Content of Sialic Acids in Newborn Rat Brain

ZHENG Zhi-Hong, LIN Ling

2000, 20(3): 129-132.

Asbtract ( 743 )

PDF (7003KB) ( 484 )

Related Articles | Metrics

We have investigated the effects of ethanol exposure in utero on the content of sialic acid and sialidase activity in newborn rat brain. Dams were exposed to ethanol in gestation days 7,8,13 and 14,the content of sialic acid were significantly increased in postnatal days 3 （PD3） and 5 （PD5） pups brain. No significant change was found on the sialidase activity.

The Influence of Mannose and Mannose-related Regents on the Binding of Mouse Sperm-zona Pellucida

HE Cai-Gu, CHEN Wen-Cheng, JIANG Yi-Ping

2000, 20(3): 133-137.

Asbtract ( 763 )

PDF (9617KB) ( 483 )

Related Articles | Metrics

To investigate the role of mannose and mannose receptor in sperm-zona binding in mouse. Capacitated spermatozoa, preincubated in the presence of D-mannose （DM） or D-mannosylated albumin （DMA），were co-incubated with zona-onclosed eggs pretreated with α-Mannosidase or Concanavalin A （ConA） respectively at the same time. The role of DM、DMA、α-Mannosidase、ConA in the interaction between mouse sperm and zona were studied by testing their effects on binding sperm to eggs. The results showed that the DM、DMA、α-Mannosidase、ConA decrease the number of sperm bound per zona pellucida. This findings suggested that mannose receptor may have a role in the interaction between sperm-zona.

Double Fluorescence Immunostaining for Tyrosine Hydroxylase and Glutamate in Rat Midbrain Sections

LIN Ling, ZHENG Zhi-Hong

2000, 20(3): 138-140.

Asbtract ( 1404 )

PDF (5356KB) ( 643 )

Related Articles | Metrics

Double fluorescence immunostain of tyrosine hydroxylase （TH） and glutamate （Glu） was used in midbrain sections of rat.With modified procedure of brain perfusion for reducing background of Glu staining,we got satisfied results,and found that 91%±4% of DA neurons were Glu positive.Cytohistological studies demonstrated that most ventral midbrain DA neurons were glutamatergic.

Application of Polymerase Chain Reaction on Detection of Mouse Hepatitis Virus

SHAO Wei-Juan, GAO Cheng, CHEN Hong-Shu, WANG Sheng-Chang, BAO Chun-Yang, WANG Xiao-Ming

2000, 20(3): 141-143.

Asbtract ( 1062 )

PDF (4680KB) ( 615 )

Related Articles | Metrics

The SCID mice、BALB/c nude mice and BALB/c mice were intraperitoneally inoculated with quantitative mixture of MHV1，MHV3，A59, JHM and euthanized on 1st，2nd，5th，8th day after inoculation. The blood and liver samples were taken,and the liver was homogenized with plastic homogenizer in a 1. 5ml microfuge tube for extracting total tissue RNA. Then, the RNA was transcribed into cDNA by using Mu-MLV re-verse transcriptase and the cDNA was amplified by PCR in same tubes.The results indicated that MHV of the blood and liver were detected on 1st day in SCID mice and BALB/c nude mice and on 5th day in BALB/c mice after inoculation.

Enriched PCR for Detection of Salmonella spp. in Mice

WANG Yue-Ling, HUANG Xiao-You, LIU Yun-Bo, TIAN Cui, HOU Jin-Xin, KANG Ai-Li, ZHU Jian, SHU Xin

2000, 20(3): 144-147.

Asbtract ( 858 )

PDF (7384KB) ( 467 )

Related Articles | Metrics

A pair of oligonucleotide primer was synthesized according to the invA gene nu-cleotide sequence within Salmonella specices. A new method of PCR was successfully developed by enrichment broth cultivation for the detection of Salmonella in laboratory animals. The DNA was extracted from 7 strains of Salmonella and 3 strains of nonsalmonella bacteria （by a rapid boiled-lysate technique）. All Salmonella yielded 284 bp specific band, while no specific product yielded in non-salmonella bacteria. As few as 50 CFU of salmonella in pure culture could be detected by PCR. Three sample-processing methods for mice feces, including low-and high-speed centrifugation, binding to glass powder,and selective enrichment cultivation, were used for the extraction of DNA template ,and the corresponding sensitivities of the methods were 2×10⁴,5×10³，10 CFU, respectively.One hundred and five laboratory animals were detected for the salmonella by enriched PCR procedure and conventional culture technique.The results suggested that the enriched cultivation-PCR have advangtages in rapid detection of Salmonella in a large number of laboratory animals.

Table of Content