Purification and Analysis of the Circulating Antigens of Schistosomiasis Japonica in Rabbits

Abstract

Abstract: The circulating antigen （CAg） of schistosomiasis japonica from the sera of Schi-stosoma japonicum-infected rabbits was purified by immune-affinity chromatography. The purified CAg exhibited seven protein bands by SDS-PAGE. The molecular weights of them are 144,118, 100，88，63，57 and 46 kD respectively. These CAgs showed five sugar protein bands by PAS colouring. The molecular weights of these sugar proteins are 144,118，100, 63 and 57 kD respectively. The CAgs released from the ova of the schistosome contain 114 and 118 kD sugar proteins as shown by ELIB.

Key words: Schistosomiasis japonica, Circulating antigen, Rabbit

Xu Guo-fang1; Peng Min2. Purification and Analysis of the Circulating Antigens of Schistosomiasis Japonica in Rabbits[J]. , 1993, 13(3): 125-128.

[1]	Honghua XU, Tian JIN, Hai WANG, Mengying SHEN, Rui WANG, Yijia ZHOU, Ying TAN. Influence of Corneal Staining in Rabbits on the Evaluation of Eye Irritation Test Results [J]. Laboratory Animal and Comparative Medicine, 2023, 43(3): 307-313.
[2]	Qingyi LUO, Tiling ZHANG, Yunchuan DING, Jian CHEN, Li ZHAO, Qinghui WANG. Establishment of a Rabbit Heart-failure Model Using Transverse Aortic Constriction [J]. Laboratory Animal and Comparative Medicine, 2022, 42(5): 401-408.
[3]	Lidan SUN, Feixia YANG, Di ZHANG, Zeliang CHEN, Zhihui ZHANG, Yingjun LI, Lijun SHI. Effectiveness and Safety of a Domestic Porcine Small Intestinal Submucosa Acellular Matrix in Rabbit Dura Mater Repairing [J]. Laboratory Animal and Comparative Medicine, 2022, 42(1): 36-41.
[4]	TANG Yi, YU Guodong, HE Xingchen, WANG Jia, ZHANG Tian. Effects of Pharyngeal Refreshing Pills on Immune Cell Subsets and NF-κB Signaling Pathway in Chronic Pharyngitis [J]. Laboratory Animal and Comparative Medicine, 2021, 41(5): 426-434.
[5]	WEN Fuli. Establishment of Eimeria stiedai Infected Rabbit Model and Nested PCR Assay [J]. Laboratory Animal and Comparative Medicine, 2020, 40(6): 477-482.
[6]	GE Jian, ZHU Caihong, WU Yanlin, WU Yongjie. Pathological Mechanism of Conjunctival Injury in Diabetic Model Rabbits [J]. Laboratory Animal and Comparative Medicine, 2020, 40(2): 136-140.
[7]	ZHOU Zhiwen, WANG Yucheng, ZHAO Lifang, CAO Jiaqi, DING Yueyou, LIU Huidong, HOU Lei, CHANG Wei. A Rabbit Model of Myocardial Infarction by Using Minimally Invasive Cardiac Catheter Technique [J]. Laboratory Animal and Comparative Medicine, 2020, 40(1): 9-14.
[8]	XU Zhenkun, REN Liwei, YUAN Tao. Establishment of Atherosclerosis Model Induced by Feeding High-fat Diet Plus Carotid Artery Balloon Injury in Rabbits with Insulin Resistance [J]. Laboratory Animal and Comparative Medicine, 2020, 40(1): 28-32.
[9]	FENG Kai, YANG Lei-xi, CHENG Yi-lin, WEI Yan-chun, TAO Ru, ZHOU Tong-xin, TANG Yi-qun. Comparison on Rabbit and Rat Atrial Fibrillation Models Induced by Intravenous Injection of Acetylcholine-Calcium Chloride Mixed Solution [J]. Laboratory Animal and Comparative Medicine, 2019, 39(5): 371-376.
[10]	XUE Ying, FAN Jiang-lin, LIU En-qi. Genetically Modified Rabbit Models for Medical Sciences [J]. Laboratory Animal and Comparative Medicine, 2019, 39(3): 169-177.
[11]	DING Lei, WANG Shou-bao, JING Hui, LI Yao. Preliminary Construction and Analysis of Right Ventricular Overload Model in Newborn Rabbits [J]. Laboratory Animal and Comparative Medicine, 2019, 39(3): 187-192.
[12]	WANG Cong, CHEN Xiao-xue, YANG Shao-ling, WANG Feng-ling, FAN Lin-yan, HE Qian-qian. Comparative Analysis of Rabbit Carotid Atherosclerosis Models Established by Collar And Air-drying Method [J]. Laboratory Animal and Comparative Medicine, 2019, 39(3): 208-212.
[13]	MI Jin-xia, FANG Zhao-qin. Research Progress of Rabbit VX2 Tumor Model [J]. Laboratory Animal and Comparative Medicine, 2019, 39(2): 163-168.
[14]	ZHOU Zhi-wen, ZHAO Li-fang, CAO Jia-qi, ZHENG Hong-chao, DING Yue-you, ZHU Fu, CHANG Wei. A Minimally Invasive Method of Electrophysiological Examination and Arrhythmia Models in Rabbits [J]. Laboratory Animal and Comparative Medicine, 2018, 38(5): 365-371.
[15]	SONG Zi-Jian, ZHENG Xin, WANG Jin, ZHANG Xing-Chen, GUO Kai-Jin. Investigation on Defect Length in a Model of Radius Critical-size Defect in Adult Rabbits [J]. Laboratory Animal and Comparative Medicine, 2018, 38(3): 182-187.

Purification and Analysis of the Circulating Antigens of Schistosomiasis Japonica in Rabbits

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments