Abstract: Objective To identify the kill effect of Microtus fortis culture supernatants against Schistosoma japonicum. Method Co-culture schistosomula with supernatants of wild Microtus fortis embryos, skin, lungs and peritoneal fibroblasts were collected at the logarithmic phase in this study. The death rate of schistosomula was observed and calculated in 96 h. We used 20% M. fortis serum as a positive control and DMEM medium as a blank control. Result After co-culture with lungs and peritoneal fibroblasts culture supernatant 72 h, schistosomula show death sign, including: shrinking skin, constriction. After 96 h, schistosomula dissolved, and the death state of the schistosomula was slightly different with the positive control group. The death rate is 8.3%±2.1%, 9.3%±1.38%, 17.2%±3.27% and 15.1%±4.35% respectively after 96h co-culture with Microtus fortis skin, embryos, peritoneal fibroblasts and lungs. Conclusion Killing effect of lungs and peritoneal fibroblasts culture supernatant of M. fortis was higher than that of the same treated skin and embryonic cell group in vitro (P<0.05).

Key words: Microtus fortis, Schistosoma japonicum, Fibroblasts, Schistosomula, Killing effect