Establishment and Evaluation of Acute Kidney Injury Mice Model by Ischemia Reperfusion and Cisplatin Induction

doi:10.3969/j.issn.1674-5817.2014.03.004

Abstract

Abstract: Objective To evaluate renal tissue damage in acute renal injury model mice established by ischemia reperfusion (I/R) with different renal ischemia times and intraperitoneal injection of cisplatin with different concentrations. Methods The male C57BL/6 mice were randomly divided into the I/R group and cisplatin induction group. All the mice were sacrificed ,whose blood were sampled for detection of serum creatinine(Scr)and urea nitrogen(Bun), the kidneys samples were dyed with HE for observation of the pathological changes and evaluation of acute tubular necrosis score(ATN score). In situ renal tubular epithelial cells apotosis were detected by TUNEL. Results The levels of BUN and Scr and ATN score in I/R group and cisplatin induction group were much higher than those of in the sham group and control group (P<0.05). As to the situation where cisplatin concentration heightened and ischemia times prolonged, the apoptosis cells gradually increased, and the pathological damage in renal tissues was more severe. Conclusion Renal tubular necrosis occurred in some of the ischemia-reperfusion 45 min group. The ideal time of acute ischemia kidney injury is 30~40 min. 10 mg/kg intraperitoneal injection twice is a suitable dose to AKI model induced by cisplatin.The degree of renal tissue injury of the mice was more severely in ischemia-reperfusion group than cisplatin induced group.

Key words: Acute kidney injury, Cisplatin, Ischemia/reperfusion, Cell apoptosis

CLC Number:

Q95-33

JIANG Yan, WANG Wei, LI Ze-zheng, CHENG Jin, WANG Wei-wei, ZHANG Jin-yuan. Establishment and Evaluation of Acute Kidney Injury Mice Model by Ischemia Reperfusion and Cisplatin Induction[J]. Laboratory Animal and Comparative Medicine, 2014, 34(3): 187-192.

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