Abstract:

Objective To track and observe whether the long-term application of micro-electrolyzed sterile water system in laboratory animal barrier facilities can continuously maintain the terminal water aseptic.Methods From April 2018 to March 2022, the microbiological detection of the micro-electrolyzed sterile water system had been tracked and monitored once each quarter in the barrier facilities of producing and breeding SPF grade rats and mice in Laboratory Animal Center of Sun Yat-sen University. Totally 8 sampling points were set, including one sampling point before the intake of the microelectrolytized water outlet into the water duct, six sampling points at the terminal water outlet, and one sampling point for the water return of the water network. Samples were taken according to aseptic operation procedures, and microelectrolysis aseptic water was detected according to the national standards GB 14925—2010 and GB/T 14926.41—2001.Results From April 2018 to March 2022, a total of 17 tests were conducted. During the normal operation of the micro-electrolyzed sterile water system, the quality of micro-electrolyzed water at 8 sampling points was sterile, in line with the national standard.Conclusion Micro-electrolyzed sterile water system can maintain the sterility of the terminal water supply, and can be used in laboratory animal barrier facilities for a long time.

Key words: Micro-electrolysis, Sterile water, Monitor, Barrier facilities, Laboratory animals