Preliminary Establishment and Research on Form Deprivation Myopia Model in Tree Shrew

doi:10.3969/j.issn.1674-5817.2017.03.001

Abstract

Abstract: Objective To establish the adolescence and early adulthood form deprivation myopia (FDM) model in tree shrew and to observe the retinal morphology, in order to explore the role of age in the development of myopia and the effection of local retinal mechanism for FDM. Method Thirty tree shrews at age of 4 months and 5 months without congenital myopia and other eye diseases were respectively selected. All tree shrews were randomly divided into: control group, and cover group. In the cover group, right eyes served as the experimental eye, left eye as control eyes. Experimental eye were covered with handmade semi-translucent film. then measure the diopter and ?axial?length of tree shrews after being covered for 3 weeks and 6 weeks. The retinal thickness and the number of cells in each layer of retina were observed by electron microscopy after being covered for 6 weeks. Results The tree shrews were born 4 months and 5 months form deprivation after 3 weeks, hyperopia was alleviated but not statistically significant compared with control eyes, and two groups of tree shrews cover eye diopter and eye axis are different obviously compared with control eyes after 6 weeks. At deprivation period, axial continue to extend and gradually increase to myopia, and they have a good negative linear relationship. Form deprivation can lead to thinning of the retina of the tree shrew, can also lead to number decrease on photoreceptor cell layer, inner nuclear layer, and ganglion cell layer cells. Conclusion Form deprivation can induce myopia formation and retinal morphological change in adolescence and early adulthood tree shrews.

Key words: Tree shrew, Form deprivation myopia (FDM), Retinal morphology

CLC Number:

Q95-33

YANG Dong-mei, ZHU Qin, LI Na, GUO Li-yun, ZHANG Xiao-fan, ZHANG Jie-ying, HU Min, DAI Jie-jie. Preliminary Establishment and Research on Form Deprivation Myopia Model in Tree Shrew[J]. Laboratory Animal and Comparative Medicine, 2017, 37(3): 171-178.

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