Antagonistic Effect of Selenium on Change of Mitochondrial Membrane Potential of NRK-52E Cells Induced by Sodium Fluoride

doi:10.3969/j.issn.1674-5817.2017.03.002

Abstract

Abstract: Objective To study effects of fluoride on the change of mitochondrial membrane potential in rat renal tubular epithelial cells (NRK-52E cells). Methods The experiments were divided into 4 groups: the control group, the fluoride group, the selenium group and the selenium intervention group.NRK-52E cells were cultured with fluoride and selenium respectively in various concentration, and fluoride and selenium combination for 48 h. (5 mg/L or 20 mg/L NaF、0.0171 mg/L or 0.0342 mg/L Na₂SeO₃). The mitochondrial membrane potential were detected by flow cytometry. The activity of enzymes and the content of free radicals were examined by relevant reagent kit, such as superoxide dismutase(SOD) reagent kit. Results Compared with the control group, the mitochondrial membrane potential decreased (P<0.05 or P<0.01) and SOD, total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-PX) and catalase (CAT) enzyme activities decreased (P<0.05) with the increase of the concentration of sodium fluoride.The mitochondrial membrane potential of the selenium intervention group was increased (P<0.01) compared with that of the fluoride exposed group.Compared with the fluoride group of 20 mg/L, the relative selenium intervention group SOD, T-AOC, GSH-PX and CAT activities were increased (P<0.01). Compared with the selenium group of 0.0171 mg/L, the relative selenium intervention group the mitochondrial membrane potential decreased (P<0.01) and SOD, T-AOC, GSH-PX and CAT enzyme activities decreased (P<0.05 or P<0.01). 5 mg/L the fluoride group compared with the selenium group, the mitochondrial membrane potential changed significantly (P<0.01). But for SOD, T-AOC, GSH-PX and CAT activities ,there were no significant changed. 20 mg/L the fluoride group compared with the selenium group, the mitochondrial membrane potential changed significantly (P<0.01). SOD, T-AOC, GSH-PX and CAT activities also changed significantly (P<0.01). Conclusion The certain concentration of selenium could induce the decrease of mitochondrial membrane potential in NRK-52E cells induced by fluoride, that may be achieved by restoring the activities of activities.

Key words: Sodium fluoride, Selenium, Rat renal tubular epithelial cells, Mitochondrial membrane

CLC Number:

Q95-33

CHANG Kai, WANG Yu, PANG Wen-biao, GAO Ji-ping, CHEN Zhao-yang, SONG Guo-hua. Antagonistic Effect of Selenium on Change of Mitochondrial Membrane Potential of NRK-52E Cells Induced by Sodium Fluoride[J]. Laboratory Animal and Comparative Medicine, 2017, 37(3): 179-184.

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