Study on Vaginal Irritation of dl-Mandelic Acid Thermosensitive in situ Gel in Miniature Pig

doi:10.3969/j.issn.1674-5817.2015.05.003

Abstract

Abstract: Objective To study the vaginal irritation of dl-Mandelic acid thermosensitive in situ Gel(dl-Mandelic acid-TISG) in miniature pigs. Methods Nine miniature pigs injected with estradiol benzoate (400 μg/kg) were randomly divided into three groups: control group, dl-Mandelic acid -TISG group and nonoxynol-9 gel (N-9 gel) group. Each miniature pig was administrated with 5 mL gel through vagina once daily for 7 days. Cervicovaginal lavage (CVL) fluids were collected before administration and at the 24 h and 72 h after the last administration. The number of inflammatory cells and neutrophilic granulocytes in the CVL were detected by the flow cytometry. The miniature pigs were sacrificed at 72 h after the last administration. Histopathological changes of the vaginal mucosa were observed under light microscope. Results The number of inflammatory cells and neutrophilic granulocytes in the dl-Mandelic acid-TISG group were significantly lower than that in the N-9 group (P<0.05). Histological scoring of vaginal mucosa showed that there were no obvious mucosal epithelium damage, vascular congestion and inflammatory cell infiltration (5.10±0.47) in the dl-Mandelic acid-TISG group.There was no significant difference (3.30±1.60, P>0.05) compared with the control group (P>0.05) but significant difference compared with the N-9 group (20.50±3.50, P<0.05). Conclusion The vaginal irritation of dl-Mandelic acid-TISG in miniature pigs is mild.

Key words: dl-Mandelic acid-TISG, Miniature pig, Inflammatory cells, Neutrophilic granulocytes

CLC Number:

Q95-33

XIA Min-jie, HUANG Chao, TIAN Fang, WANG Yu-zhu, ZHI Rui-na, DING Xun-cheng, Yan Qian-Qin, LI Wei-hua. Study on Vaginal Irritation of dl-Mandelic Acid Thermosensitive in situ Gel in Miniature Pig[J]. Laboratory Animal and Comparative Medicine, 2015, 35(5): 356-361.

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